if a 10-3 virus dilution put on agar using the methods you will read about (and would have used) in the lab manual resulted in > 2000 plaques (which really are not countable as they are too crowded) on the final agar plate; and the 10-4 virus dilution gave you 200 plaques; and the 10-5 virus suspension dilution gave you 20 plaques, you need to then figure out what your final dilution factor was and use that to calculate the virus titer (i.e., the number of virus particles per ml) in the ORIGINAL suspension.
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if a 10-3 virus dilution put on agar using the methods you will read about (and...
0.2 ml of a 10-9 dilution of a bacterial suspension plated on an agar plate gave rise to 156 distinct colonies. What is the concentration (CFU/ml) of bacteria in the original, undiluted suspension?
For the plaque assay, your T4 bacteriophage titer is 7.8x108 pfu/mL. You do six 1:10 dilutions of the virus, putting 10µL of each dilution in a tube of top agar with 100µL of E. coli. After pouring the contents of the top agar onto an LA plate, you incubate overnight. How many plaques would you expect to see on the final plate?
Dilutions. In the following example: a. What is the titer of the virus? b. Is the virus titer high enough of the newly arrived virus shipment to be used as a control in the experiment? c. Show your work Note: The virus titer needed as a control in the experiment was at least 1 x 10^7 viruses per ml. The control virus stock arrived from the ATCC distributor arrived frozen and was labeled as 3x10^8 viruses/ml (equal to 300,000,000 viruses/ml...
Please show how to do these enumeration questions by showing the work. What is the dilution factor if you add 625 µL of culture to 4.375 mL of dilution medium? You count the 10-3 dilution of a yeast suspension using the 1/25 mm2 boxes : you find 45 cells in box 1, 15 cells in box 2, 42 cells in box 3, 23 cells in box 4, and 26 cells in box 5. a) What is the original concentration of...
You spread 0.1 mL volume of a 10^(-5) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 223 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? (2 points) B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-7)...
You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? (2 points) B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8)...
(A) You have a sample with an original concentration of 1.0 x 10^7 CFU/mL. With the Plate Count Method, what final dilution factor would be needed to produce countable plates? Show your work. (B) Describe a dilution scheme (how many tubes, what volume in each tube, what DF is achieved in each step) that uses only the 9-mL blank diluent tubes to achieve the dilution needed for this FDF.
please help with whatever possible. thank you so much in advance. Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
3. You are measuring the growth of bacterium using cfu mL·You collect samples at various times and dilute them in 10-fold series (as shown in the 2 row of the table) and plate 0.1 ml of each of these dilutions on five standard lab agar plates. The average number of colonies per plate are below Time of Colonies on plate at each dilution ars 45 290 TNTC TNTC 195 20 TNTC TNTC 200 TNTC too numerous to count ADB MO...
3. You are measuring the growth of bacterium using cfu mL·You collect samples at various times and dilute them in 10-fold series (as shown in the 2 row of the table) and plate 0.1 ml of each of these dilutions on five standard lab agar plates. The average number of colonies per plate are below Time of Colonies on plate at each dilution ars 45 290 TNTC TNTC 195 20 TNTC TNTC 200 TNTC too numerous to count ADB MO...