Question

G,B,D,E t would you need to evaluate the effectiveness of the seven antibiotics tested as shown in the picture shown? what t would needto evaluate he effectivemess of tno 7 antibiotics tested Satellite colonies are sometimes observed when the Kirby-Bauer disk diffusion method is used (see image below). What do you think the presence of satellite colonies indicates?

Answer 1

2. Satellite colonies appear when the pH is below 7. Satellite colonies are very small colonies of cells that have not taken up the plasmid that form around a large colony that has taken up the bla-containing plasmid. The satellites form because the beta-lactamase released by the bla-expressing colony degrades the ampicillin in the vicinity of the colony.

1. You have to test according to kirby bauer method. Tests for antibiotic sensitivity include: Kirby-Bauer method. Small wafers containing antibiotics are placed onto a plate upon which bacteria are growing. If the bacteria are sensitive to the antibiotic, a clear ring, or zone of inhibition, is seen around the wafer indicating poor growth.