You performed a co-immunoprecipitation experiment to identify cellular proteins associated with protein X. For the experiment,...
Parkin Mannosidase II Merge Parkin Y-Adaptin Merge Parkin BIP Merge Parkin LAMP-1 Merge Parkin Ubiquitin Merge FIG. 2. Immunocytochemical detection of overexpressed Par- kin. COS-7 cells were transfected with FLAG-Parkin and subjected to co-immunostaining with anti-FLAG antibody (green) along with the Golgi complex marker mannosidase II (A), trans-Golgi network marker yadaptin (B), endoplasmic reticulum marker BIP/GRP78 (C), and ly- sosomal marker LAMP-1 (D), all with red fluorescence. E, COS-7 cells transfected with FLAG-Parkin were immunostained with anti-FLAG antibody (green) and...
(17) Suppose that you are planning an immunofluorescent histochemical staining experiment to determine if protein X is expressed in the same cells as protein Y. From the table below, select appropriate pairs of primary and secondary antibodies that would likely be most effective for this purpose. See emission spectra in the diagram to the right for reference. TRITO TR (A) Rabbit anti-X; Rabbit anti-Y // FITC-Goat anti-rabbit IgG; TR-Goat anti-rabbit IgG (B) Mouse anti-X; Rabbit anti-Y // TRITC-Donkey anti- mouse...
16. You have cloned a centromere from yeast. You have also identified three genes, X, Y and Z that code for proteins that are a subset of the kinetochore proteins that are bound to the centromere. None of your three genes is essential but each does contribute to the overall efficiency of chromosomal segregation. You raise antibodies to each of your three proteins. You also contstruct four strains, a wild-type strain, a strain in which gene X is deleted (XD),...