Question

NA Biology: Lab 5 & 6___________________________________________________________________

List steps to set up a gel and how to run it (electrophoresis)

Buffer, gel, wells, lane, band

How to visualized DNA and how EtBr (ethidium bromide) works – intercalation

Factors that affect how far the DNA runs (6 main factors)

Know important abbreviation and nomenclature used: SNP, RFLP, RE, EcoRI, bp, kb

Describe and know relationships between: DNA, Palindromes, SNP, RFLP, RE

Identify a palindrome and how many pieces of DNA would result from RE digest given a palindrome

What is and how to do a ‘DNA RE digestion’

How to compare digestion patterns and match samples (“Who done it”?)

Measure distance of a band and purpose of a standard curve

Mitosis, Meiosis, & Inheritance: Lab 7_______________________________________________________

Definitions: interphase (steps) vs mitosis vs meiosis

Meiosis – steps, get four distinct haploid cell with ½ as many chromosomes

Mitosis – steps, get two identical diploid cell with the same number of chromosomes

Interphase – G1, S, G2 – growth and DNA synthesis

Identify and draw stages of mitosis in whitefish or onion root tip

Know definitions: genotype, phenotype, allele, homozygous, heterozygous, dominant, recessive

Define and differentiate Mendel’s Laws of Inheritance

WT (wild-type) vs mutant, from genotypic AND phenotypic standpoint

Perform monohybrid cross given phenotype/genotype data (what we did in class)

Given a numbers of individuals, determine if the trait is sex-linked or autosomal

Be able to sex fruit flies – “black butts”

Identify: WT, white eyes, dumpy wings, yellow bodies, and the other wing mutation in fruit flies

Tissues: Lab 8_________________________________________________________________________

Know general structure, function, composition, location (if applicable) of each type of tissue

see Tissues Study Guide

Be able to find the epithelial layer on a slide with multiple layers of tissue.

Be able to identify the type of tissue (general or specific) presented on a microscope.

Ex. Simple cuboidal vs stratified squamous vs transitional vs nerve

Know definitions: tissue, histology, apical, basal

Know the terms for the possible cell shapes and layers in epithelial tissue (don’t forget transitional and pseudostratified).

Be able to give a verbal description or sketch them.

Know the terms (define): lacuna, chondrocyte, matrix, osteon, lamella, canaliculi, central canal, microvilli, goblet cells, adipocyte, striations, and be able to identify them on a slide.

Be able to identify the type of muscle cell (skeletal, cardiac, smooth) on a slide.

Explain the roles of collagen and elastic fibers in connective tissue.

Be able to identify a neuron on a slide.

Answer 1

STOCK SOLUTIO GATHER MATERIALS<-CASTING HATER! E QUIPMENT →SAFETY ELECTROPHORECIS PREPARE STOCK SOLUONSi TAE CAST THE GEL PLACE GEL INSIbE →TURN FRONT DIAL ATTACH СО㎍TO PLATE POUR HOT SOLUTION INTO YEL PLATE DAIT FOR COLUTION TO SOLI DIFY LOAD SAMPLES TO GEL PROCESS GEL PHoTO GRA PH USING UVCAMERA

2) Ethedium bromides interchelates itself between the double helix of DNA- and can be visualised by UV light. Therefore it is used in electrophoresis. UV light camera is used to visualize DNA.

3) a) Charge of DNA
b) Size of DNA
c) Voltage of electric field
d) Adsorption of substance on medium
e) pH of buffer used.
f) Molecular slieving

4) SNP - Single nucleotide polymorphisms
RFLP - Restriction fragment length polymorphism
RE - Restriction endonuclease
EcoRI - ESCHERICHIA COLI RI
bp- base pair
kb - kilo bases