Question

15. In which of the following procedures does the sample stop moving until conditions change? i. Size exclusion chromatography ii. SDS-electrophoresis iii. Isoelectric Focusing iv. Density gradient centrifugation V. Rate-zonal centrifugation a. ii, iii, and v. b. iii, and iv. c. ii, iv, and v. d. only iii e. all excepti. 16. or the following types of separation, which gives one the best chance of obtaining a protein in its properly folded state? a. Affinity Chromatography b. SDS Electrophoresis c. Partition Coefficient Chromatography d. Size Exclusion Chromatography e. Ion Exchange Chromatography

Answer 1

15.) @ all exceft i [Only in case of size exclusion chromatography you need not to change condition as samples does elutes once loaded without any external application to change the condition] 16) 6 SDS elechophoresis [ Non-denaturing Condition for protein purification is electrophorests, Since the intactmess on the structural integsh of the isolated prolein is remain unchanged throughout the series of processes intend to solate one of a new pokon from Complex mixture using elechophorests