Homework Answers
32. to make a protein of large size to move faster in SDS gel-electrophoresis system:
Ans. D. decrease the acrylamide concentration of the gel
As lower the percentage of acrylamide the pore size will increases which helps in migration of bigger proteins.
33. The following statements are accurate:
Ans. C. i, ii and iii
SDS binds to proteins, introduces negative charge according to the length of the proteins. SDS is a an detergent which is involved in solubilizing the membrane. And, it unfold proteins and neutralizes hydrogen bonds.
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32. Which of the following would make a protein of a large size move faster through...
Exercise IV. Fill in the Blank 1. The method of Centrifugation, polyacrylamide gel electrophoresis, western blotting,...
Exercise IV. Fill in the Blank 1. The method of Centrifugation, polyacrylamide gel electrophoresis, western blotting, affinity purification) is the most widely used technique for determining the approximate molecular weight of a protein. 2. (Centrifugation, affinity chromatography, sonication, gel electrophoresis) is a method in which macromolecules are separated due to their size, charge, and other physical properties 3. SDS-PAGE is a form of electrophoresis in the presences of a/an (acidic solution, basic solution, anionic detergent, cationic detergent). 4. SDS not...
15. In which of the following procedures does the sample stop moving until conditions change? i....
15. In which of the following procedures does the sample stop moving until conditions change? i. Size exclusion chromatography ii. SDS-electrophoresis iii. Isoelectric Focusing iv. Density gradient centrifugation V. Rate-zonal centrifugation a. ii, iii, and v. b. iii, and iv. c. ii, iv, and v. d. only iii e. all excepti. 16. or the following types of separation, which gives one the best chance of obtaining a protein in its properly folded state? a. Affinity Chromatography b. SDS Electrophoresis c....
parts a,b, c please 3. Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis a. After pouring the...
parts a,b, c please
3. Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis a. After pouring the SDS polyacrylamide gel, you realize that instead of 2 ml, you used 4 ml of 30% acrylamide/bisacrylamide solution for the preparation of the separating gel (in both cases for 5 ml, total gel volume). How would this affect the separation of your proteins during SDS PAGE? Explain your answer! b. You have to remake the gel and are now making sure just to add...
(iln 2. Use the letters given to answer the questions below for full credit Protein name pl A Non...
(iln 2. Use the letters given to answer the questions below for full credit Protein name pl A Nonheme iron-containing fermitin (Pf) B Pynuvate: ferredoxin oxidoreductase, alpha Subunit (PorA) 44613 CGTP-binding proteia (Gtpl) D Co-chaperone (GiroES) E Adhesin-thiol peroxidase (TagD) FI Fumarase (Fund) 19,155 5.49 5.81 40,444 5.53 12,860 6.59 18,161 8.18 50,844 7.29 i) (5 points) Sketch the Isoelectric focusing gel after the separation of above proteins is performed. Clearly label everything needed for full credit. i) (5 points)...
a. Which spot corresponds to the protein with the largest molecular weight? b. When pH = 7, what order would the follow...
a. Which spot corresponds to the protein with the largest
molecular weight?
b. When pH = 7, what order would the following peptides elute
from a column filled with cation exchange resin? Size exclusion
column?
Protein 1 – 400 kDa;
10%A 5%E, 5%F, 10% G, 5% H, 10%I, 10%K, 10%L 10%R, 5%P, 5%S, 10%W,
5%Y
Protein 2: 40 kDa
5%A, 5%C, 5%D, 5%E, 5%F, 10%G, 5%I, 10%K, 5%L, 5%M, 5%N, 10%P, 5%R,
5%S, 5%T, 5%V, 5%W,
Protein 3 – 175...
Which of the following statements are true about protein domains? I. Polypeptide chains longer than 200...
Which of the following statements are true about protein domains? I. Polypeptide chains longer than 200 amino acids in length have multiple domains. II. Domains are tertiary structural components of proteins. III. Different domains are encoded by different exons of a gene. IV. Each domain in a protein has a specific function. A. I, II, III, and IV B. I, II, and III C. II, III, and IV D. II and IV E. I and IV
For the following questions, consider six proteins, each 50 amino acids long, made of just a single type of amino acid....
For the following questions, consider six proteins, each 50
amino acids long, made of just a single type of amino acid. Protein
P is made of proline; K, lysine; S, serine; E. glutamic acid; F,
phenylalanine.; and G. glycine. Your answers are going to be P, K,
S, E, F or G.
1. Which of these proteins would move fastest on SDS-PAGE?
2. Which of these proteins would move fastest on Sephadex?
3. Which of these proteins would move closest...
A Which of the following statements about Electromobility Shift Assays (EMSA) is correct? 1 EMSA gels...
A Which of the following statements about Electromobility Shift Assays (EMSA) is correct? 1 EMSA gels contain SDS detergent to maintain the shape of the of protein. 2 Large DNA molecules flow faster down the gel towards the positive pole in EMSA. 3 EMSA gels cannot be used to determine whether a particular protein has bound to a specific DNA sequence. 4 Smaller DNA molecules flow faster down the gel towards the positive pole in EMSA. B Which of the...
5 and 6 please 5. Which of the following make up the three major subclassifications of...
5 and 6 please
5. Which of the following make up the three major subclassifications of lipids? Amino acids and proteins ii. Mono-, di-, and polysaccharides iii. Fats, oils, and phospholipids iv. Waxes V. Steroids a. i, ii, and iii b. i, iii, and iv c. ii, iii, and iv d. iii, iv, and v 6. Which of the following are the two types of nucleic acids in all living systems? i. Deoxyribonucleic acid ii. Gluconucleic acid iii. Ribonucleic acid...
Which of the following are accurate descriptions of buffer solutions? 1. If a solution is buffered, its pH is nearl...
Which of the following are accurate descriptions of buffer solutions? 1. If a solution is buffered, its pH is nearly neutral. II. The mechanism by which buffered solutions resist pH changes is not well understood. III. Addition of strong acid to a buffer shifts the equilibrium such that Ht is consumed. IV. If strong base is added to a buffer, it reacts with the acid component of the mixture. V. Buffers only work well if the one conjugate is present...
Exercise IV. Fill in the Blank 1. The method of Centrifugation, polyacrylamide gel electrophoresis, western blotting, affinity purification) is the most widely used technique for determining the approximate molecular weight of a protein. 2. (Centrifugation, affinity chromatography, sonication, gel electrophoresis) is a method in which macromolecules are separated due to their size, charge, and other physical properties 3. SDS-PAGE is a form of electrophoresis in the presences of a/an (acidic solution, basic solution, anionic detergent, cationic detergent). 4. SDS not...
15. In which of the following procedures does the sample stop moving until conditions change? i. Size exclusion chromatography ii. SDS-electrophoresis iii. Isoelectric Focusing iv. Density gradient centrifugation V. Rate-zonal centrifugation a. ii, iii, and v. b. iii, and iv. c. ii, iv, and v. d. only iii e. all excepti. 16. or the following types of separation, which gives one the best chance of obtaining a protein in its properly folded state? a. Affinity Chromatography b. SDS Electrophoresis c....
parts a,b, c please
3. Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis a. After pouring the SDS polyacrylamide gel, you realize that instead of 2 ml, you used 4 ml of 30% acrylamide/bisacrylamide solution for the preparation of the separating gel (in both cases for 5 ml, total gel volume). How would this affect the separation of your proteins during SDS PAGE? Explain your answer! b. You have to remake the gel and are now making sure just to add...
(iln 2. Use the letters given to answer the questions below for full credit Protein name pl A Nonheme iron-containing fermitin (Pf) B Pynuvate: ferredoxin oxidoreductase, alpha Subunit (PorA) 44613 CGTP-binding proteia (Gtpl) D Co-chaperone (GiroES) E Adhesin-thiol peroxidase (TagD) FI Fumarase (Fund) 19,155 5.49 5.81 40,444 5.53 12,860 6.59 18,161 8.18 50,844 7.29 i) (5 points) Sketch the Isoelectric focusing gel after the separation of above proteins is performed. Clearly label everything needed for full credit. i) (5 points)...
a. Which spot corresponds to the protein with the largest
molecular weight?
b. When pH = 7, what order would the following peptides elute
from a column filled with cation exchange resin? Size exclusion
column?
Protein 1 – 400 kDa;
10%A 5%E, 5%F, 10% G, 5% H, 10%I, 10%K, 10%L 10%R, 5%P, 5%S, 10%W,
5%Y
Protein 2: 40 kDa
5%A, 5%C, 5%D, 5%E, 5%F, 10%G, 5%I, 10%K, 5%L, 5%M, 5%N, 10%P, 5%R,
5%S, 5%T, 5%V, 5%W,
Protein 3 – 175...
For the following questions, consider six proteins, each 50
amino acids long, made of just a single type of amino acid. Protein
P is made of proline; K, lysine; S, serine; E. glutamic acid; F,
phenylalanine.; and G. glycine. Your answers are going to be P, K,
S, E, F or G.
1. Which of these proteins would move fastest on SDS-PAGE?
2. Which of these proteins would move fastest on Sephadex?
3. Which of these proteins would move closest...
5 and 6 please
5. Which of the following make up the three major subclassifications of lipids? Amino acids and proteins ii. Mono-, di-, and polysaccharides iii. Fats, oils, and phospholipids iv. Waxes V. Steroids a. i, ii, and iii b. i, iii, and iv c. ii, iii, and iv d. iii, iv, and v 6. Which of the following are the two types of nucleic acids in all living systems? i. Deoxyribonucleic acid ii. Gluconucleic acid iii. Ribonucleic acid...
Which of the following are accurate descriptions of buffer solutions? 1. If a solution is buffered, its pH is nearly neutral. II. The mechanism by which buffered solutions resist pH changes is not well understood. III. Addition of strong acid to a buffer shifts the equilibrium such that Ht is consumed. IV. If strong base is added to a buffer, it reacts with the acid component of the mixture. V. Buffers only work well if the one conjugate is present...
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