Question

Exercise IV. Fill in the Blank 1. The method of Centrifugation, polyacrylamide gel electrophoresis, western blotting, affinity purification) is the most widely used technique for determining the approximate molecular weight of a protein. 2. (Centrifugation, affinity chromatography, sonication, gel electrophoresis) is a method in which macromolecules are separated due to their size, charge, and other physical properties 3. SDS-PAGE is a form of electrophoresis in the presences of a/an (acidic solution, basic solution, anionic detergent, cationic detergent). 4. SDS not only(folds proteins more compactly, completely unfolds proteins, cut proteins into smaller fragments, separates proteins), but also gives all the proteins a (negative, neutral, positive) charge. Calibration of the gel with protein labeling, marker proteins) allows us to estimate the molecular weight of unknown proteins. 5. (immunofluorescent markers, antibodies, 6. The sieving properties of the gel are determined by the (density concentration, surface area) of the gel. 7. The range of separation of proteins is usually given in (kDauD, nD, number of amino acids). 8. Immunoblotting is also called Northern blotting, Southern blotting, Western blotting, Immunohistochemistry) Western blotting first begins by transferring the amino acids, protein bands) from the gel onto a charged plate, cellulose sheet, nitrocellulose sheet) 9. (antibodies, nucleotides (filter paper, negatively 10. Once the blotting is complete, the next step is to treat it with coomassie blue&hematoxylin staining, primary & secondary antibodies, TBST & transfer buffer). Pg. 5

Answer 1

Question: 1. The method of ..........(Centrifugation, polyacrylamide gel electrophoresis, western blotting, affinity purification) is the most widely used technique for determining the approximate molecular weight of a protein.

Answer: Polyacrylamide gel electrophoresis (PAGE)

Question: 2. (Centrifugation, affinity chromatography, sonication, gel electrophoresis) is a method in which macromolecules are separated due to their size, charge, and other physical properties

Answer: Gel electrophoresis

Question: 3. SDS-PAGE is a form of electrophoresis in the presences of a/an (acidic solution, basic solution, anionic detergent, cationic detergent).

Answer: An anionic detergent

Sodium dodecyl sulfate (SDS) is an anionic detergent.