Question

A Which of the following statements about Electromobility Shift Assays (EMSA) is correct?

1 EMSA gels contain SDS detergent to maintain the shape of the of protein.

2 Large DNA molecules flow faster down the gel towards the positive pole in EMSA.

3 EMSA gels cannot be used to determine whether a particular protein has bound to a specific DNA sequence.

4 Smaller DNA molecules flow faster down the gel towards the positive pole in EMSA.

B Which of the following statements about eukaryote transcription is correct?

1 RNA polymerase II transcribes RNA for ribosomes.

2 RNA polymerase III is found in nucleoli.

3 RNA polymerase II transcribes the mitochondrial genes into mRNA, r RNA and tRNA.

4 RNA polymerase III transcribes RNA precursors for ribosomes.

5 RNA polymerase I synthesises the RNA precursors of rRNA.

Answer 1

Ans- 1 EMSA gels contain SDS detergent to maintain the shape of the of protein. -- False

2) 2 Large DNA molecules flow faster down the gel towards the positive pole in EMSA.- False

3 EMSA gels cannot be used to determine whether a particular protein has bound to a specific DNA sequence.--- False

4 Smaller DNA molecules flow faster down the gel towards the positive pole in EMSA. --- True