Question

To study the function of the essential cytosolic Hsc70 genes in yeast, researchers constructed a shuttle vector in which a copy of the Hsc70 gene was ligated to the GAL1 promoter. The vector was then introduced into haploid yeast cells in which all four copies of the Hsc70 genes had been disrupted. Following introduction of the vector, you would expect that:

		the yeast cells would grow on galactose but not glucose medium.
		the yeast cells would grow on glucose but not galactose medium.
		the yeast cells would grow on either glucose or galactose medium.
		on transfer to either glucose or galactose medium, the vector-carrying cells would eventually stop growing because of insufficient Hsc70 activity. A haplotype is a set of closely linked genetic markers on a particular chromosome that tend to be inherited together. The genetic technique that looks at inheritance patterns and uses haplotypes in determining gene locations is: candidate gene approach all are correct linkage disequilibrium mapping linkage mapping To inactivate the function of a wild-type small GTPase one could introduce: an RNAi to silence the expression of the guanine nucleotide exchange factor. Cre recombinase. Cas9. a dominant negative allele of a GTPase gene that binds to and inactivates a guanine nucleotide exchange factor.

Answer 1

* The correct option is Option (A) the yeast cells would grow on galactose but not glucose medium.

In a haploid yeast, when a shuttle vector (in which a copy of Hsc70 gene has been ligated to GAL1 promoter) is introduced, the bacteria now has the ability to grow on galactose medium because of metabolic intake of galactose in it whereas it can't intake glucose when placed in a glucose medium and therefore can't grow in a glucose containing culture medium. Hence, the result.

* The correct option is Option (C) Linkage disequilibrium mapping.

Linkage disequilibrium mapping (also known as Association Mapping) is the genetic technique that looks at inheritance patterns and uses haplotypes in determining gene locations.

* The correct options are Option (B) and Option (D).

To inactivate the function of a wild-type small GTPase, one can introduce -

1. Cre recombinase (tyrosine recombinase biocatalyst abstracted from P1 bacteriophage)

2. A dominant negative allele of a GTPase gene that binds to and inactivates a guanine nucleotide exchange factor.