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In molecular biology, what's the meaning of the terms "downstream signaling" and "upstream signaling"? What's the...

In molecular biology, what's the meaning of the terms "downstream signaling" and "upstream signaling"? What's the difference between them?

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If you think of a transcription factor, than this factor needs to be regulated. This happens either by signalling pathways and also on the gene expression level (how much of the factor is available). So everything what regulates this factor is called upstream.

Everything which is regulated by our factor (other genes) is located downstream of it. I think these terms come from the flow diagrams which are pretty usual in this context.

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