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NAME: Preparation of Buffers and Titration of Amino Acid Use the formula weights of chemicals used in this laboratory in answering the questions below. Use 6.86 as pKa2 for phosphate buffer. For questions 1 and 2, write the formula of each ingredient and not or 1. Determine the mass of ingredients to be mixed to prepare 0.5 L of a 0.1 M phosphate buffer of pH 6.86. 2. Calculate the volume of ingredients that would be needed to prepare 1.0 liter of a 0.2 M acetate buffer of pH 5.2. 3. The pKa of a weak acid is 5.0. Calculate the pH of the buffer containing this acid, when the ratio of proton acceptor A to proton donor HA is equal to: a. 1.0 20
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