Question

Polymerase chain reaction (PCR): The gold standard of nucleic acid amplification The polymerase chain reaction (PCR) is a powerful tool for amplifying genetic material (that is, making many copies of it). This technique can be used for a wide range of applications, from examining and manipulating the specific genes involved in making L. monocytogenes pathogenic to analyzing the phylogenetic relationships between microbes. What are the steps of PCR required to amplify the recombinant DNA? Drag and drop the events into the proper sequence from left to right.

Answer 1

Step 1 DNA extracted from sample to be tested.

step 2 sample is heated to denature double stranded DNA.

step 3 sample is cooled to allow oligonucleotides used as Primers to anneal to DNA.

step 4 sample is heated and DNA polymerase extendspe primers using original DNA as template

step 6 cycles of heating and cooling repeated 20-30 Times

step 7 product is visualised through electrophoresis

step 8 Probe is used to identify particular sequence of interest known to exist in the microbe of interest.