Question

41. Which of the following statements about protein-ligand binding is correct? A) The K is equal to the concentration of ligand when all of the binding sites are occupied. B) The K is independent of such conditions as salt concentration and pH. C) The larger the K. (association constant), the weaker the affinity. D) The larger the K. the faster is the binding. E) The larger the K, the smaller the K. (dissociation constant) 42. The ability of O, to bind hemoglobin a. is less in the T state compared to the R state b. is affected by steric hindrance within hemoglobin c. depends on the concentration of CO,, although O, and CO, do not compete for a binding site d. all of the above e, none of the above 43. In plots of Oz-binding curves for hemoglobin under these conditions, which one would be to the left of all others? a. pH 7.2, 8 mM BPG b. pH 7.4, 5 mM BPG c. pH 7.2, 3 mM BPG d. pH 7.2,5 mM BPG c. pH 7.4, 3 mM BPG 44. Hill coefficient, the slope of the line in the Hill plot, a. cannot indicate the presence of any allosteric effects b. equals the total number of ligands bound to a protein c. cannot be less than 1.0 d. can never be 0.0 e. None of the above 45. In the binding of oxygen to myoglobin, the relationship between the concentration of oxygen and the fraction of binding sites occupied can best be described as: A) hyperbolic. B) linear with a negative slope. C) linear with a positive slope. D) random E) sigmoidal 46. The a-keratin chains indicated by the diagram below have undergone one chemical step. To alter the shape of the keratin chains-as in hair waving-what subsequent steps are required? A) Chemical oxidation and then shape remodeling B) Chemical reduction and then chemical oxidation C) Chemical reduction and then shape remodeling D) Shape remodeling and then chemical oxidation E) Shape remodeling and then chemical reduction

Answer 1

week 29 Day 199-167 08.00 09.00 10.00 11.00 12.00 Boluhan Pub * 20 protein ligand complex cue want greater binding affinity on the ligand for its target a this can be achieved at lower Kd valve so lower Kd valve have greater affinity bindina of the ligand for its turgel & the larger the la valve, the more wearely the target molecule and ligand are attracted to and bind to one 6 ᏬH : ao low ka valve have high apnity interachon becauce it takes less ligand to fill half the protein 8 & we lenow, ka ar Kd ka - association constant S lcd = disso crahen constant 16.00 so if led is smaller then ka is larger 17.00 so for protein - ligand finding have largen ka value & smalley led valve. ophon (e) is correct. 13.00 14.00 15.00 16.00 18.00 Started with CamScanner