1.
DNA-binding proteins are proteins that bind to single- or double-stranded DNA, generally in the major groove if the binding is sequence-specific – as with transcription factors that regulate expression of genes, and nucleases that cleave DNA between nucleotides whereas,
DNA cleavage is a reaction that severs one of the covalent sugar-phosphate linkages between NUCLEOTIDES that compose the sugar phosphate backbone of DNA. It is catalyzed enzymatically, chemically or by radiation. Cleavage may be exonucleolytic - removing the end nucleotide, or endonucleolytic - splitting the strand in two.
Nowadays, antimicrobial peptides are widely recognized as a class of promising candidates with activity against multidrug-resistant bacteria
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DNA cleavage activity linked to anti cancer properties because the mechanism of amplification of DNA cleavage can be explained by assuming that binding of amplifier changes the DNA conformation to allow anticancer drug to interact more appropriately with the specific sequences, resulting in enhancement of anticancer effect.
1. what is the difference between DNA binding and DNA cleavlage 2. why is DNA cleavage...
18. What is the difference between hemolytic cleavage and heterolytic cleavage?
1. what are the differences between DNA replication vs. PCR? 2. From gel electrolysis, what does the DNA strand look like? 3. Why is cancer a genetic disease but are not inherited?
The binding difference between carbon and oxygen molecules is the same as 2, the binding energy is 118 kcal/mole and the carbon is 144 kcal/mole. Why is carbon larger than oxygen? please explain based on MO theoory
(3a)What is the difference between a binding price ceiling and a binding price floor in a market for a resource? (3b)What is the difference between a non-binding price ceiling and a binding price ceiling in a market for a resource? (3c)What is the policy objective of a government in setting a price ceiling or a price floor in a market for a resource? (3d)With the use of clearly labeled demand-supply diagrams show the difference between the concepts of (a) a...
1. What is the source of the “spacer” DNA in the bacterial CRISPR locus? 2. Why does the cas9 enzyme have two nuclease domains? 3. What is the relationship between the tracrRNA and the regularly interspersed repeats of the CRISPR locus? Why is this important? 4. In the lab version of the CRISPR/cas9 system, what is the “chimeric RNA”?
1. A biochemist is attempting to separate a DNA-binding protein (protein X) from other proteins in a solution. Only three other proteins (A, B, and C) are present. The proteins have the following properties: pl (isoelectric point) Size Mr Bind to DNA? protein A 7.4 protein B 3.8 protein C 7.9 protein X7.8 82,000 21,500 23,000 22,000 yes yes no yes What type of protein separation techniques might she use to protein X from the other proteins. Give a flow...
1. Explain the difference between an intron and an exon. 2. Why do the two possible PCR products differ in size by 300 base pairs? 3. Explain how agarose electrophoresis separates DNA fragments. Why does a smaller DNA fragment move faster than a larger one? 4. What kind of controls are run in this experiment? Why are they important? Could others be used?
DNA and RNA: A. One difference between RNA and DNA is the pentose sugar. What characteristics are affected by the type of sugar in the molecule? B. Why can there be unusual base pairings in RNA, but with DNA it has to be GC and AT base pairs only?
17. What is the difference between determinant and indeterminate cleavage? Text 18. You will notice that most animals have spiral and Text cleavage or radial and Text cleavage. 19. What are six categories of data used in constructing the phylogenetic tree showing the diversification of Animals? Text 20. Use the phylogenetic trees to answer these questions. Which phylum or phyla... a. form the clade of animals? b. forms the basal taxon for animals? c. includes animals with a backbone? d....
Describe 2 reasons why the DNA major groove offers more specificity for protein binding.