Question

1. what is the difference between DNA binding and DNA cleavlage

2. why is DNA cleavage activity linked to anti cancer properties while DNA binding activity is linked to anti bacterial properties

3 what is the importance of DNA cleavage studdies

Answer 1

1.

DNA-binding proteins are proteins that bind to single- or double-stranded DNA, generally in the major groove if the binding is sequence-specific – as with transcription factors that regulate expression of genes, and nucleases that cleave DNA between nucleotides whereas,

DNA cleavage is a reaction that severs one of the covalent sugar-phosphate linkages between NUCLEOTIDES that compose the sugar phosphate backbone of DNA. It is catalyzed enzymatically, chemically or by radiation. Cleavage may be exonucleolytic - removing the end nucleotide, or endonucleolytic - splitting the strand in two.

Nowadays, antimicrobial peptides are widely recognized as a class of promising candidates with activity against multidrug-resistant bacteria

2.

DNA cleavage activity linked to anti cancer properties because the mechanism of amplification of DNA cleavage can be explained by assuming that binding of amplifier changes the DNA conformation to allow anticancer drug to interact more appropriately with the specific sequences, resulting in enhancement of anticancer effect.