1. What is the source of the “spacer” DNA in the bacterial CRISPR locus? 2. Why...
1. What is the source of the “spacer” DNA in the bacterial CRISPR locus?
2. Why does the cas9 enzyme have two nuclease domains?
3. What is the relationship between the tracrRNA and the regularly interspersed repeats of the CRISPR locus? Why is this important?
4. In the lab version of the CRISPR/cas9 system, what is the “chimeric RNA”?
Homework Answers
The Source of Spacer DNA in The Bacterial CRISPER Locus is Previous Exposure To Foreign DNA .
Examples are -a virus or plasmid. Means when previously a Foreign DNA is exposed then it act as a
Spacer when repetetion occures.
Add Answer to:
1. What is the
source of the “spacer” DNA in the bacterial CRISPR locus?
2. Why...
1. The CRISPR-Cas system in bacteria is most similar to what system in vertebrates? A. Immune...
1. The CRISPR-Cas system in bacteria is most similar to what system in vertebrates? A. Immune system B. Circulatory system C. Nervous system D. Digestive system 2. You are using CRISPR-Cas technology to introduce a single nucleotide change in a gene of interest in living cells. You are designing your experiment. In which component of the reaction will you engineer the single nucleotide change? A. Donor DNA B. Spacer RNA C. Repeat RNA D. tracrRNA 3. What is sgRNA? A....
4. The CRISPR-Cas9 system is an important new technique in molecular biology. What is the natural...
4. The CRISPR-Cas9 system is an important new technique in
molecular biology. What is the natural function of this system?
Describe how you would use this system to generate a null mutation
in another organism (i.e. explain Figure 6-43). How does it work?
What is the modification of the method that allows for correction
of a mutation (e.g. the mouse crystalline gene)? And lastly, what
are the problems with the CRISPR system?
FIGURE 6-43 Single-nucleotide mutations
can be introduced into...
Question 1: What was determined to be the function of the CRISPR loci in microbes? Question...
Question 1: What was determined to be the function of the CRISPR loci in microbes? Question 2: Explain the differences in the roles of the cas7 and cas9 gene. Question 3: What genetic material does CRISPR target? Question 4: Due to the ability of CRISPR to cleave DNA sequences at specific sites, it is considered a programmable version of what? Question 5: Define and explain the significance of the PAM sequence. Question 6: What is the role of tracrRNA in...
please help answer these questions 3. What are the similarities, differences, advantages, and disadvantages of CRISPR-based...
please help answer these questions
3. What are the similarities, differences, advantages, and disadvantages of CRISPR-based gene editing versus Zinc-finger nucleases and TALENS? 4. What is crRNA and what does it do? 5. What is tracrRNA and what does it do? 6. What is the PAM sequence and what is its significance? 7. What can nuclease-deficient Cas9 (acas) proteins be used for? 8. You want to insert DNA encoding an epitope tag to the end of a specific gene you...
Please help me answering these multiple questions of how the CRISPR system works, thank you! 1....
Please help me answering these multiple questions of how the CRISPR system works, thank you! 1. Match the CRISPR component with its function. (matching the upper case letters to the lower case letters) A). Cas9 B). gRNA (sometimes called sgRNA) C). Non-homologous End Joining (NHEJ) D). Homology-directed Repair (HDR) a). A short RNA molecule that guides a DNA cutting enzyme to a specific location in genomic DNA. b). A type of DNA repair mediated by a set of enzymes all...
Please have a different answer than the other post, and use layman's terms as well hhmi...
Please have a different answer than the other post, and use
layman's terms as well
hhmi Biolnteractive Using CRISPR to identify the Functions of Butterfly Genes Activity Student Handout This document is made available by the Howard Hughes Medical Institute. Using this document, you agree to use this document in accordance with the Terms of Use. INTRODUCTION Scientists have determined the complete DNA sequences of the genomes for many organisms, including humans. By analyzing patterns in those sequences, they can...
1. what is CRISPR gene editing? 2. what enzyme is used to cut DNA? 3.Why do...
1. what is CRISPR gene editing? 2. what enzyme is used to cut DNA? 3.Why do bacteria use CRISPR? 4.How is the enzyme targeted to a specific DNA sequence? 5.what need to be done in order to make sure the proper human protein is made in bacteria cells? 6.How can you check for proper orientation of the inserted DNA in the plasmid? 7. Why are iPSCs useful for studying hereditary neuronal diseases such as Alzheimer's. 8. How are pig cells...
ASAP please .. Hey, I need help answering these questions, about the introduction of an article...
ASAP please ..
Hey, I need help answering these questions, about the introduction
of an article about HIV inhibition using adenovirus-delivered
CRISPR/CAS9. Thaanks!!
strand, resulting in double-stranded breaks (DSBs) that trigger cellular repai mechanisms. In eukaryotes, the DSBs are more commonly repaired by the mechanism ot error prone non ho mologous end joining (NHEJ), therefore generating sequence changes, for instance insertions and deletians (indels), around the DSBs. Owing to the simplicity of manipulation and versati ity, the CRISPR/Cas9 system has been...
PRE-LAB QUESTIONS 1. List three structural differences between DNA and RNA 2. Why is it necessary...
PRE-LAB QUESTIONS 1. List three structural differences between DNA and RNA 2. Why is it necessary to heat the DNA source and the buffer mixture?
1. what is the difference between DNA binding and DNA cleavlage 2. why is DNA cleavage...
1. what is the difference between DNA binding and DNA cleavlage 2. why is DNA cleavage activity linked to anti cancer properties while DNA binding activity is linked to anti bacterial properties 3 what is the importance of DNA cleavage studdies
4. The CRISPR-Cas9 system is an important new technique in
molecular biology. What is the natural function of this system?
Describe how you would use this system to generate a null mutation
in another organism (i.e. explain Figure 6-43). How does it work?
What is the modification of the method that allows for correction
of a mutation (e.g. the mouse crystalline gene)? And lastly, what
are the problems with the CRISPR system?
FIGURE 6-43 Single-nucleotide mutations
can be introduced into...
please help answer these questions
3. What are the similarities, differences, advantages, and disadvantages of CRISPR-based gene editing versus Zinc-finger nucleases and TALENS? 4. What is crRNA and what does it do? 5. What is tracrRNA and what does it do? 6. What is the PAM sequence and what is its significance? 7. What can nuclease-deficient Cas9 (acas) proteins be used for? 8. You want to insert DNA encoding an epitope tag to the end of a specific gene you...
Please have a different answer than the other post, and use
layman's terms as well
hhmi Biolnteractive Using CRISPR to identify the Functions of Butterfly Genes Activity Student Handout This document is made available by the Howard Hughes Medical Institute. Using this document, you agree to use this document in accordance with the Terms of Use. INTRODUCTION Scientists have determined the complete DNA sequences of the genomes for many organisms, including humans. By analyzing patterns in those sequences, they can...
ASAP please ..
Hey, I need help answering these questions, about the introduction
of an article about HIV inhibition using adenovirus-delivered
CRISPR/CAS9. Thaanks!!
strand, resulting in double-stranded breaks (DSBs) that trigger cellular repai mechanisms. In eukaryotes, the DSBs are more commonly repaired by the mechanism ot error prone non ho mologous end joining (NHEJ), therefore generating sequence changes, for instance insertions and deletians (indels), around the DSBs. Owing to the simplicity of manipulation and versati ity, the CRISPR/Cas9 system has been...
PRE-LAB QUESTIONS 1. List three structural differences between DNA and RNA 2. Why is it necessary to heat the DNA source and the buffer mixture?
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