Question

1. The CRISPR-Cas system in bacteria is most similar to what system in vertebrates?

A. Immune system

B. Circulatory system

C. Nervous system

D. Digestive system

2. You are using CRISPR-Cas technology to introduce a single nucleotide change in a gene of interest in living cells. You are designing your experiment. In which component of the reaction will you engineer the single nucleotide change?

A. Donor DNA

B. Spacer RNA

C. Repeat RNA

D. tracrRNA

3. What is sgRNA?

A. Donor RNA and tracr RNA linked together

B. tracrRNA and crRNA linked together

C. Donor RNA and crRNA linked together

D. tracrRNA with a DNA tag

4. Which part of the CRISPR-Cas system binds the target DNA?

A. tracrRNA

B. crRNA

C. Cas9

D. Cas2

Answer 1

1.) (A) CRISPR -Cas system in bacteria is most similar to immune system of vertebrates as it is a bacterial immune system which keeps the memory of the previous phage infection , to combat the infection if infected by the same type of phage again .

2.) (A) Single nucleotide change will be engineered in DONOR DNA as it will be going to incorporate as an action of homologous end joining DNA damage repair system.

3.)(B) sgRNA is an abbreviation for “single guide RNA.” As the name implies, an sgRNA is a single RNA molecule that contains both the custom-designed short crRNA sequence fused to the scaffold tracrRNA sequence.

4.) (B) crRNA binds to target DNA .CRISPR RNAs (crRNAs) had an antisense function
against phage or plasmid transcripts, thereby anneals to it's counterpart or target genetic element.

Answer 2

Circular system