All of the following are required for genome editing with the CRISPR-Cas 9 system except: A....
All of the following are required for genome editing with the CRISPR-Cas 9 system except:
A. Cas9 nuclease
B. An sgRNA
C. A repair mechanism for a double-strand DNA break (DSB)
D. Invading DNA protospacers
E. A PAM sequence
Homework Answers
PAM sequence is the found in the DNA sequence not in cas
protein.
Add Answer to:
All of the following are required for genome editing with the
CRISPR-Cas 9 system except:
A....
Which of the following statements is FALSE? In the context of CRISPR-Cas9 gene editing system, the...
Which of the following statements is FALSE? In the context of CRISPR-Cas9 gene editing system, the PAM sequence: a. A sequence adjacent to the target DNA sequence to be cleaved b. Is present on the non-complementary target DNA strand to the crRNA c. Has the sequence “5’-NGG-3’” where N is any nucleotide d. Stands for "protospacer adjacent motif" e. Is present in the bacterial genome
4. The CRISPR-Cas9 system is an important new technique in molecular biology. What is the natural...
4. The CRISPR-Cas9 system is an important new technique in
molecular biology. What is the natural function of this system?
Describe how you would use this system to generate a null mutation
in another organism (i.e. explain Figure 6-43). How does it work?
What is the modification of the method that allows for correction
of a mutation (e.g. the mouse crystalline gene)? And lastly, what
are the problems with the CRISPR system?
FIGURE 6-43 Single-nucleotide mutations
can be introduced into...
CRISPR-Cas9 provides an unprecedented means of modifying the genome of almost any organism. Which of the...
CRISPR-Cas9 provides an unprecedented means of modifying the genome of almost any organism. Which of the following component(s) of this system provide(s) the specificity of the endonucleolytic cleavage? the sgRNA the PAM sequence Cas9 a and b a, b and c
please help answer these questions 3. What are the similarities, differences, advantages, and disadvantages of CRISPR-based...
please help answer these questions
3. What are the similarities, differences, advantages, and disadvantages of CRISPR-based gene editing versus Zinc-finger nucleases and TALENS? 4. What is crRNA and what does it do? 5. What is tracrRNA and what does it do? 6. What is the PAM sequence and what is its significance? 7. What can nuclease-deficient Cas9 (acas) proteins be used for? 8. You want to insert DNA encoding an epitope tag to the end of a specific gene you...
Copy of In the CRISPR/Cas9 genome editing system, the job of the guide RNA is to......
Copy of In the CRISPR/Cas9 genome editing system, the job of the guide RNA is to... o cut the target sequence The job of the guide RNA is not fully known yet. o identify the correct sequence to be cut o help the plasmid enter the cells hide the plasmid from the immune system
Question 38 Which of the following statements regarding CRISPR-Cas system is TRUE? Not yet answered Points...
Question 38 Which of the following statements regarding CRISPR-Cas system is TRUE? Not yet answered Points out of 2.50 Select one: a. CRISPR-Cas system uses a DNA-directed nuclease to degrade viral RNA P Flag question O b. CRISPR-Cas system uses an RNA-directed nuclease to degrade viral DNA C. CRISPR-Cas system uses a RNA-directed nuclease to degrade viral RNA O d. CRISPR-Cas system uses a DNA-directed nuclease to degrade viral DNA e. CRISPR-Cas is a defense mechanism in eukaryotes against viral...
1. The CRISPR-Cas system in bacteria is most similar to what system in vertebrates? A. Immune...
1. The CRISPR-Cas system in bacteria is most similar to what system in vertebrates? A. Immune system B. Circulatory system C. Nervous system D. Digestive system 2. You are using CRISPR-Cas technology to introduce a single nucleotide change in a gene of interest in living cells. You are designing your experiment. In which component of the reaction will you engineer the single nucleotide change? A. Donor DNA B. Spacer RNA C. Repeat RNA D. tracrRNA 3. What is sgRNA? A....
Please help me answering these multiple questions of how the CRISPR system works, thank you! 1....
Please help me answering these multiple questions of how the CRISPR system works, thank you! 1. Match the CRISPR component with its function. (matching the upper case letters to the lower case letters) A). Cas9 B). gRNA (sometimes called sgRNA) C). Non-homologous End Joining (NHEJ) D). Homology-directed Repair (HDR) a). A short RNA molecule that guides a DNA cutting enzyme to a specific location in genomic DNA. b). A type of DNA repair mediated by a set of enzymes all...
ASAP please .. Hey, I need help answering these questions, about the introduction of an article...
ASAP please ..
Hey, I need help answering these questions, about the introduction
of an article about HIV inhibition using adenovirus-delivered
CRISPR/CAS9. Thaanks!!
strand, resulting in double-stranded breaks (DSBs) that trigger cellular repai mechanisms. In eukaryotes, the DSBs are more commonly repaired by the mechanism ot error prone non ho mologous end joining (NHEJ), therefore generating sequence changes, for instance insertions and deletians (indels), around the DSBs. Owing to the simplicity of manipulation and versati ity, the CRISPR/Cas9 system has been...
7.. In the controlled termination method of DNA sequencing, reading the gel from _____ gives the...
7.. In the controlled termination method of DNA sequencing, reading the gel from _____ gives the sequence in the _____ direction; _____ fragments that were terminated _____ in polymerization move faster down the gel a. bottom to top; 5′ to 3′; shorter; early b. top to bottom; 5′ to 3′; longer; early c. bottom to top; 5′ to 3′; longer; later d. top to bottom; 5′ to 3′; shorter; early e. bottom to top; 3′ to 5′; shorter; early 8....
4. The CRISPR-Cas9 system is an important new technique in
molecular biology. What is the natural function of this system?
Describe how you would use this system to generate a null mutation
in another organism (i.e. explain Figure 6-43). How does it work?
What is the modification of the method that allows for correction
of a mutation (e.g. the mouse crystalline gene)? And lastly, what
are the problems with the CRISPR system?
FIGURE 6-43 Single-nucleotide mutations
can be introduced into...
please help answer these questions
3. What are the similarities, differences, advantages, and disadvantages of CRISPR-based gene editing versus Zinc-finger nucleases and TALENS? 4. What is crRNA and what does it do? 5. What is tracrRNA and what does it do? 6. What is the PAM sequence and what is its significance? 7. What can nuclease-deficient Cas9 (acas) proteins be used for? 8. You want to insert DNA encoding an epitope tag to the end of a specific gene you...
Copy of In the CRISPR/Cas9 genome editing system, the job of the guide RNA is to... o cut the target sequence The job of the guide RNA is not fully known yet. o identify the correct sequence to be cut o help the plasmid enter the cells hide the plasmid from the immune system
Question 38 Which of the following statements regarding CRISPR-Cas system is TRUE? Not yet answered Points out of 2.50 Select one: a. CRISPR-Cas system uses a DNA-directed nuclease to degrade viral RNA P Flag question O b. CRISPR-Cas system uses an RNA-directed nuclease to degrade viral DNA C. CRISPR-Cas system uses a RNA-directed nuclease to degrade viral RNA O d. CRISPR-Cas system uses a DNA-directed nuclease to degrade viral DNA e. CRISPR-Cas is a defense mechanism in eukaryotes against viral...
ASAP please ..
Hey, I need help answering these questions, about the introduction
of an article about HIV inhibition using adenovirus-delivered
CRISPR/CAS9. Thaanks!!
strand, resulting in double-stranded breaks (DSBs) that trigger cellular repai mechanisms. In eukaryotes, the DSBs are more commonly repaired by the mechanism ot error prone non ho mologous end joining (NHEJ), therefore generating sequence changes, for instance insertions and deletians (indels), around the DSBs. Owing to the simplicity of manipulation and versati ity, the CRISPR/Cas9 system has been...
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