Homework Answers
7 answer
Reverse primer
5'CAGTTTCGACCCTATAGCGCGCCTCAGGACAACGTACTACTGTCCAGTACCTATGGGGACTTGCAAAAGCCTTTGGCATATCCAATCAGGGCCATGCAATTTAATGATGGACAGGCATTACTTGAGTTACACAGTTACGAATGGCAGTAC3'
8 answer
Forward primer is same as plus strand
5'GTACTGCCATTCGTAACTGTGTACTCAAGTAATGCCTGTCCATCATGTAAATTGCATGGCCCTGATTGGATATGCCAAAGGCTTTTGCAAGTCCCCATAGGTACTGGACAGTAGTACGTTGTCCTGAGGCGCGCTATAGGGTCGAAACTG3'
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please help me!!!!!!!!!
do both i need help
Dz. You are creating PCR primers for the...
To do the PCR, we used 2 universal primers that roughly match sequences near the 5’...
To do the PCR, we used 2 universal primers that roughly match sequences near the 5’ and 3’ ends of the 16S rRNA gene. The forward primer binds near the 5’ end to the complementary strand of the sequences shown below. The sequence of the forward primer is: Agagtttgatcctggctcag The reverse primer binds near the 3’ end to the sequences shown below. The sequence of the reverse primer is: ACGGCTACCTTGTTACGACTTG To find the primer in the sequence below, you must...
Please help with all questions. I provided all the information that I have. The sequence below...
Please help with all questions. I provided all the information that I have. The sequence below represents the genomic DNA sequence of the first 440 bp of your gene of interest (exon 1 in blue). You want to amplify this full 440 bp region by PCR, for cloning into a plasmid vector. tgaagtccaactcctaagccagtgccagaagagccaaggacaggtacggctgtcatcacttagacctcaccctgtggagccacaccctagggttggccaatctactcccaggagcagggagggcaggagccagggctgggcataaaagtcagggcagagccatctattgcttacatttgcttctgacacaactgtgttcactagcaacctcaaacagacaccatggtgcatctgactcctgaggagaagtctgccgttactgccctgtggggcaaggtgaacgtggatgaagttggtggtgaggccctgggcaggttgctatcaaggttacaagacaggtttaaggagaccaatagaaactgggcatgtggagacagagaagactcttgggtttctgataggcactgactctctctgcctattggtctattttcccaccc 1.1 Design a 20 nucleotide forward & reverse primer set that will allow you to amplify the sequence above. (note - primers should be at the beginning...
please help me with both of these questions!!!! 3:33 PM Wed Mar 18 Enter answer... 5:06...
please help me with both of these questions!!!!
3:33 PM Wed Mar 18 Enter answer... 5:06 Exit You are trying to amplify an eye color gene in Drosophila. Based on the DNA sequence for the gene, you design the following primers: Forward Primer: 5' GCATGCTGAG CCTAGTAACT 3 Reverse Primer: 5' CTTAAAGCTT ACTGGTCAAC 3' True or false? These are good primers to use in PCR. Hint: use the formula: Tm (in C) = 4(G+C) + 2(A + T) True False ormula:...
please help im lost!!! please help me with 3,4,5 D 3. True or false? The Taq...
please help im lost!!! please help me with 3,4,5
D 3. True or false? The Taq Polymerase used in PCR is heat-sensitive. True [Select] False D 4. The reverse primer attaches to the select) prime end of the strand. D 5. The forward primer attaches to the [Select prime end of the strand. PCR primers should: Select all that apply
pls help The DNA sequence below is 300 bases long. This is only one strand of...
pls help
The DNA sequence below is 300 bases long. This is only one strand of DNA going from 5' starting at base 1081 to 3' ending at base 1380. The complementary strand is NOT shown. The sequence is broken up into 10 base sections to make counting easier. Design primers to amplify a DNA fragment that is 150bps in length. 1081 cagtatcagg tggtggcccc ttgcccccag tcagcaccct gacatcactg cacagtctgt 1141 ctgcctcgcc tgctccccac catggactca toatgacctc cctgcccagc gtcatgagtc 1201 tgggagagtc ctctctcctc ataggtcaaa ccgtacctgt...
Now. you should be able to answer the following questions: • How the amplification will be...
Now. you should be able to answer the following questions: • How the amplification will be done? - How you will determine your target sequence? How the amplification will be specific for certain segment? What are the requirements to carry PCR? • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5'-CTACCTGCGGGTTGACTGCTACCTTCCCGGGATGCCCAAAATTCTCGAG-3+ +3'-GATGGACGCCCAACTGACGATGGAAGGGCCCTACGGGTTTTAAGAGCTC-5'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is...
1. Create the primers to amplify the gene of interest. 1. Below is almost the full...
1. Create the primers to amplify the gene of interest. 1. Below is almost the full sequence of the coding strand of the F9 gene (exon 2-exon4) that you found in the online database (NCBI, Genomic sequence F9 gene). Some of the sequence is missing, but you know how many nucleotides are skipped. Design forward and reverse primers 18nt each for PCR that will isolate EXACTLY the sequence that is in bold typeface. Exon 2 = 164 nt Intron 2...
4. (1.5 pts)You are practicing designing primers that you can use in PCR reactions. You want...
4. (1.5 pts)You are practicing designing primers that you can use in PCR reactions. You want your primers to allow you to amplify the sequence found below. 5°-АсттсслтАTстсТАЛААТАССАТCGATСтстсссссстАGстAСCТААССАGAGACCСТАССG-3. 3-тслАсстатАсAGATTTTATсстасстаGлCAссссССATCCATCGAтTСстстстасGAТСCС-5. Markers part (a) part (b) part ck Right primer should anneal to this region Left primer should 87 nts anneal to this region 80 nts 70 nts 60 nts 50 nts 40 nts 27 nts Draw into the following gel lanes what size(s) of PCR products you would get if you...
• 4. How many copies of the primers do you add to the PCR? You add...
• 4. How many copies of the primers do you add to the PCR? You add 3 pl. of a 5 M solution of each primer, If you had 100 copies of the template DNA would there be enough primers to support 30 cycles of PCR? Remember the amount of template doubles (in theory) with each cycle. After 1 cycle there will be 2xthe starting amount, after 2 cycles 4 x the starting amount.
Can you answer and explain it please Q. You want to PCR amplify the underlined/bold DNA...
Can you answer and explain it please
Q. You want to PCR amplify the underlined/bold DNA fragment. Design two primers for this job. The size of each primer is 18 bp -3 Forward primer: 5'- Reverse primer: 5'-( ATGACTATAGGGAACACTACGAGTGTGACAACGGTACGTAAC GCGGCGATCGAGCG ACCGAA GCTACTCCGCCTTTAGCTCTCGACAAT ATCGACGCCGCTA CGGATGTGTTTAGGCCTGGT GA TGCTGG AACCCTTTA TTCGCCGAGGAAG ATGTTATCAGCGGGTCTATCAGCATCA CGATCATCGTGAGCT GGTTCTTGGCTATTTCGGAGCAGGT GTCGAATCCGATGCT ITTGTGGGTGAGATGGGTTTATTCA GTGAAGTC ATCTTACGTACACGGA ACTCAGTGTGAATTGG AAGGC AGTTTAGCGAGTGATGCGCCGCGGATTTTGTATGCGATAGGTAT GCAGTTATCTAAGCGTTTATTGCTTACCAC
please help me with both of these questions!!!!
3:33 PM Wed Mar 18 Enter answer... 5:06 Exit You are trying to amplify an eye color gene in Drosophila. Based on the DNA sequence for the gene, you design the following primers: Forward Primer: 5' GCATGCTGAG CCTAGTAACT 3 Reverse Primer: 5' CTTAAAGCTT ACTGGTCAAC 3' True or false? These are good primers to use in PCR. Hint: use the formula: Tm (in C) = 4(G+C) + 2(A + T) True False ormula:...
please help im lost!!! please help me with 3,4,5
D 3. True or false? The Taq Polymerase used in PCR is heat-sensitive. True [Select] False D 4. The reverse primer attaches to the select) prime end of the strand. D 5. The forward primer attaches to the [Select prime end of the strand. PCR primers should: Select all that apply
pls help
The DNA sequence below is 300 bases long. This is only one strand of DNA going from 5' starting at base 1081 to 3' ending at base 1380. The complementary strand is NOT shown. The sequence is broken up into 10 base sections to make counting easier. Design primers to amplify a DNA fragment that is 150bps in length. 1081 cagtatcagg tggtggcccc ttgcccccag tcagcaccct gacatcactg cacagtctgt 1141 ctgcctcgcc tgctccccac catggactca toatgacctc cctgcccagc gtcatgagtc 1201 tgggagagtc ctctctcctc ataggtcaaa ccgtacctgt...
Now. you should be able to answer the following questions: • How the amplification will be done? - How you will determine your target sequence? How the amplification will be specific for certain segment? What are the requirements to carry PCR? • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5'-CTACCTGCGGGTTGACTGCTACCTTCCCGGGATGCCCAAAATTCTCGAG-3+ +3'-GATGGACGCCCAACTGACGATGGAAGGGCCCTACGGGTTTTAAGAGCTC-5'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is...
1. Create the primers to amplify the gene of interest. 1. Below is almost the full sequence of the coding strand of the F9 gene (exon 2-exon4) that you found in the online database (NCBI, Genomic sequence F9 gene). Some of the sequence is missing, but you know how many nucleotides are skipped. Design forward and reverse primers 18nt each for PCR that will isolate EXACTLY the sequence that is in bold typeface. Exon 2 = 164 nt Intron 2...
4. (1.5 pts)You are practicing designing primers that you can use in PCR reactions. You want your primers to allow you to amplify the sequence found below. 5°-АсттсслтАTстсТАЛААТАССАТCGATСтстсссссстАGстAСCТААССАGAGACCСТАССG-3. 3-тслАсстатАсAGATTTTATсстасстаGлCAссссССATCCATCGAтTСстстстасGAТСCС-5. Markers part (a) part (b) part ck Right primer should anneal to this region Left primer should 87 nts anneal to this region 80 nts 70 nts 60 nts 50 nts 40 nts 27 nts Draw into the following gel lanes what size(s) of PCR products you would get if you...
• 4. How many copies of the primers do you add to the PCR? You add 3 pl. of a 5 M solution of each primer, If you had 100 copies of the template DNA would there be enough primers to support 30 cycles of PCR? Remember the amount of template doubles (in theory) with each cycle. After 1 cycle there will be 2xthe starting amount, after 2 cycles 4 x the starting amount.
Can you answer and explain it please
Q. You want to PCR amplify the underlined/bold DNA fragment. Design two primers for this job. The size of each primer is 18 bp -3 Forward primer: 5'- Reverse primer: 5'-( ATGACTATAGGGAACACTACGAGTGTGACAACGGTACGTAAC GCGGCGATCGAGCG ACCGAA GCTACTCCGCCTTTAGCTCTCGACAAT ATCGACGCCGCTA CGGATGTGTTTAGGCCTGGT GA TGCTGG AACCCTTTA TTCGCCGAGGAAG ATGTTATCAGCGGGTCTATCAGCATCA CGATCATCGTGAGCT GGTTCTTGGCTATTTCGGAGCAGGT GTCGAATCCGATGCT ITTGTGGGTGAGATGGGTTTATTCA GTGAAGTC ATCTTACGTACACGGA ACTCAGTGTGAATTGG AAGGC AGTTTAGCGAGTGATGCGCCGCGGATTTTGTATGCGATAGGTAT GCAGTTATCTAAGCGTTTATTGCTTACCAC
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