• 4. How many copies of the primers do you add to the PCR? You add...

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• 4. How many copies of the primers do you add to the PCR? You add 3 pl. of a 5 M solution of each primer, If you had 100 cop

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Answer 1

Answer #1

Two primers are used in each PCR reaction, and they are designed so that they flank the target region (region that should be copied). That is, they are given sequences that will make them bind to opposite strands of the template DNA, just at the edges of the region to be copied.

After 25 cycles, what began as a single molecule of DNA has been amplified into more than a billion copies 2^25. With PCR, it is routinely possible to amplify enough DNA from a single hair follicle for DNA typing.

So we would have enough primers

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Answer 2

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