Question

1. (4) The following are the results from an interrupted mating experiment. Your supervisor has asked you to interpret these results and draw a map showing the distances between the genes.
The experimental set up was as follows: Hfr
arg + mal + met + pur + pyr + xyl + azi s X F - arg- mal- met- pur- pyr- xyl- azir
Exconjugants were plated on medium containing no arginine, maltose as the sole carbon source, and azide at a selective concentration. Replica plates were then made to assess each colony’s genotype at the other loci.

*THIS IS ALL THE INFO GIVEN IN THE Q

100 xyl pyr* met Percent of Hfr genotypes among arg', mal', azi exconjugants pur* 10 20 30 1 40 50 60 Time (min)

Answer 1

Chromosome transfer in this case Hrf to F- I'd slow.

It takes 90minutes to transfer the entire chromosomes (approximately).

Here kitchen blender conjugation process is used.

To identify gene distance it is based on comp rising the entry time with interrupted mating time.

initialization of this conjugation starts from 15minute.the time gap is not equally same there is less or more distances in the graph.