Question

1. (4) The following are the results from an interrupted mating experiment. Your supervisor has asked you to interpret these results and draw a map showing the distances between the genes.
The experimental set up was as follows: Hfr
arg + mal + met + pur + pyr + xyl + azi s X F - arg- mal- met- pur- pyr- xyl- azir
Exconjugants were plated on medium containing no arginine, maltose as the sole carbon source, and azide at a selective concentration. Replica plates were then made to assess each colony’s genotype at the other loci.

*THIS IS ALL THE INFO GIVEN IN THE Q

100 xyl pyr* met Percent of Hfr genotypes among arg', mal', azi exconjugants pur* 10 20 30 1 40 50 60 Time (min)

Answer 1

SOLUTION:-

1.(4) GIVEN THAT...

1.(4) The following are the results from an interrupted mating experiment. Your supervisor has asked you to interpret these results and draw a map showing the distances between the genes. The experimental set up was as follows: Hfr argt mat met purt pyrt xyl az X F-arg mat met pur pyr xyt azi Exconjugants were plated on medium containing no arginine, maltose as the sole carbon source, and azide at a selective concentration. Replica plates were then made to assess each colony's genotype at the other loci. 12 min 100 E xyz Xyl pyr 3 min met Pyr Percent of Hfr genotypes among arg, mal, azi' exconjugants pur 6 min Pur 7 min Met 10 20 30 40 50 60 Time (min)
Since the medium do not contain arginine, maltose as sole carbon source and azide as selection marker, so these genes need to be transfered first to enable the bacterial colonies to survive on the medium then followed by xyl,pyr,met,pur genes.

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