Question

Determine :

1. number of bulge loops and internal loops,

2.the number of nucleotides in the multi-branch loop, and the degree of “sharpness” to the hairpin loops.

3.how many complementary base pairs are in the (complete) secondary structure? If a base pair in a stem is between nucleotides that are also in a loop, include that base pair in the count. 


-G-C A-V c-G-C 50 0 05-0566 0-0-0-0-0-0- 5 -A-U -

Answer 1

1. zero bulge loop and internal loop

hairpin loop internal loop multibranch loop Bulge loop

2. 22 nucleotides are there in multibranch loop. A , U, G and C are the nucleotides. Sharpness indicates rigidity of the structure.the sharpness here is 30 and 50 .

3. 16 complementary base pairs in the secondary structure.