Question

please help me with both of these questions!!!!

3:33 PM Wed Mar 18 Enter answer... 5:06 Exit You are trying to amplify an eye color gene in Drosophila. Based on the DNA sequence for the gene, you design the following primers: Forward Primer: 5' GCATGCTGAG CCTAGTAACT 3 Reverse Primer: 5' CTTAAAGCTT ACTGGTCAAC 3' True or false? These are good primers to use in PCR. Hint: use the formula: Tm (in C) = 4(G+C) + 2(A + T) True False

Answer 1

And a true Ғото 64 d рорея .. 5 cc Atect GAccc 14 стест за o Tome 4 cate) + 2(THA) 24410) & 240) 2 40+20 Reverse primer 5 GTT AMAGOTT ACTGGTC AAC 3 Tomo 418) + 2(12) 2 32+ 24 2 56°c. I priners for a PCR should be between 1825 nucleotides in length and they should have Ia çc condent between 40-604. oder - The 31 end of the primer should exact match to the template ONA - primer pairs have similar Tomin with a maximum diff exence of soc. and they should not be complen entary to each other. - so, thise dare a good primer & 2P

Ans lo- Type of that would requence. are changes in a not change DNA the sequence size of the 0 as Inversion - chromosome reary angement in which a segment of chromosome reversed. - It occurs whes a single chromosome und ergues breakage and reary angement within it rell. - ② single nucleotide change - Due to point mutation or substitution mutation. - Due to substitution mutation - single change in amino acid of protein happens which leads to malfunction. This two categones of changes generally not change the size de sequence of any olence