Please answer both parts a and b. Please explain your answer. (The marked answers in the pictures are incorrect!)
a)
b)
Homework Answers
Both primer pair B and C will bind to the given template and give a PCR product. But only Primer B will a product of 20 nucleotides.
B- As compared to the wild type, 1 migrated more. 2 migrated less and 3 migrated the same length as that of wild type.
The fragment which are larger than the wild type will migrate less as compared to the wild type. It is only possible if the 2 is larger than the wild type. DNA becomes larger due to the insertion of some bases.
So in the case of 2, there is insertion.
So correct answer is option D- insertion.
In the case of 1, there is the deletion of some nucleotides, such that its length become shorter than the wild type.
3 migrate the same length as that of wild -type. It means that either there is no deletion or insertion or there is a mutation in just one nucleotide so that the length of 3 is the same as that of wild-type. So 3 is either transversions, transition or single nucleotide polymorphism.
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Please answer both parts a and b. Please explain your answer.
(The marked answers in the...
You isolate three E. coli mutants that are unable to synthesize histidine. You amplify the gene...
You isolate three E. coli mutants that are unable to synthesize histidine. You amplify the gene responsible for the phenotype from the wildtype (WT) and mutant strains (1, 2, and 3) using PCR and run the PCR products on an agarose gel. Based on the results, which mutant is likely to have an deletion in the gene of interest? (The top of the gel is where the DNA was loaded - the negatively charged end) Wt 1 2 3
Can you explain the answers below ? Below is the same gene from page 1. Alternmative...
Can you explain the answers below ?
Below is the same gene from page 1. Alternmative splicing of the primary RNA transcript will include either exon 4 or exon 5. Assume that mutants 1 and 5 are deletion mutants; mutant 2 is a frameshift mutation; and mutant 3, 4, and 6 are substitution mutations. PCR primers are designated with arrows and lowercase letters (a, b, c, d). mutant 4 mutant 61mutant 3 KR Kpnl Kpn ATG TAG TAA exon 1...
The most common mutation occuring in people with cystic fibrosis is the loss of a single...
The most common mutation occuring in people with cystic fibrosis is the loss of a single amino acid (phenylalanine) at position 508 of the protein. This mutation O a. is a point mutation b. is an single nucleotide polymorphism c. is a deletion of 3 nucleotides d. in an insertion of a single nucleotide e. is a chromosomal rearrangement → Moving to another question will save this response. In Concience in 5 min you. For ence ne
please help me with both of these questions!!!! 3:33 PM Wed Mar 18 Enter answer... 5:06...
please help me with both of these questions!!!!
3:33 PM Wed Mar 18 Enter answer... 5:06 Exit You are trying to amplify an eye color gene in Drosophila. Based on the DNA sequence for the gene, you design the following primers: Forward Primer: 5' GCATGCTGAG CCTAGTAACT 3 Reverse Primer: 5' CTTAAAGCTT ACTGGTCAAC 3' True or false? These are good primers to use in PCR. Hint: use the formula: Tm (in C) = 4(G+C) + 2(A + T) True False ormula:...
Answers are C,A. Please explain the method to fix this kind of questions. Thanks 18. Shown...
Answers are C,A. Please explain
the method to fix this kind of questions. Thanks
18. Shown below are the results of a series of coinfections of E. coli B using T4 rll- strains similar to those employed by Benzer in his deletion mapping experiment. Each strain contains a different deletion mutation. The ability to produce wild-type progeny phage is indicated by (+), (O) indicates no wild-type progeny. + + OC Which order of deletions is most consistent with this data?...
NEED HELP WITH THESE QUESTIONS. PLEASE ANSWER ALL AND EXPLAIN AS WELL. THANKSSSSSSS 1. You want...
NEED HELP WITH THESE QUESTIONS. PLEASE ANSWER ALL AND EXPLAIN AS
WELL. THANKSSSSSSS
1. You want to clone a gene from a donor vector to a host vector. List the correct order of events in the process of cloning a. Perform ligation reaction of cloned gene and host vector. b. Perform double digestion of both donor and host vectors with the 2 restriction enzymes c. Examine donor and host vectors for restriction sites d. Purify cloned gene from donor vector...
very lost on this whole worksheet and i dont know if my current answers are even...
very lost on this whole worksheet and i dont know if my
current answers are even right, please help i need this done by
today
Name: Cloning Worksheet chromosome qut 756bp pBiochem 5.983 bp ladder Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 BamHI (4233) (230) IT Enzyme recognition S ..GATATC. 51 EcoRV S .. . 3..CTATAC... 5 12 Pvull 89 BamHI Pett You are given the task to clone yfg into the plasmid pBioChem. Follow...
Note whether in the following strains of E. coli, B-galactosidase will be: (A) Abundant in both...
Note whether in the following strains of E. coli,
B-galactosidase will be:
(A) Abundant in both the presence and absence of lactose
(B) Abundant only in the absence of lactose
(C) Abundant only in the presence of lactose
(D) Abundant only in the presence of sucrose
(D) Absent in both the presence and absence of lactose
Can you answer and explain these please?
What is the correct gene order and which 2 genes are most closely linked? (4 Points) o+at...
1) Imagine that you are interested in studying the enzyme dihydrofolate reductase (DHFR) from Mycobacterium tuberculosis...
1) Imagine that you are interested in studying the enzyme dihydrofolate reductase (DHFR) from Mycobacterium tuberculosis (this enzyme is a potential drug target). The amino acid sequence of the protein is: MTMVGCIWAQATSGVIGRGGDIPWRLPEDQAHFREITMGHTIVMGRRTWDSLPAKVRPLPGRRNWL SRQADFMASGAEWGSLEEALTSPETWVIGGGQVYALALPYATRCEVTEVDIGLPREAGDALAPVLD ETWRGETGEWRFSRSGLRYRLYSYHRS The DNA sequence within the M. tuberculosis genome that codes for the protein is: ATGACGATGGTGGGGCTGATCTGGGCTCAAGCGACATCGGGTGTCATCGGCCGCGGCGGCGACATCCCCT GGCGCTTGCCCGAGGACCAGGCGCATTTCCGGGAGATCACCATGGGGCACACGATCGTGATGGGCCGGCG CACATGGGATTCGCTGCCGGCTAAAGTCCGGCCGCTGCCCGGCCGGCGAAATGTCGTACTGAGCCGCCAA GCTGACTTTATGGCCAGCGGGGCTGAGGTTGTCGGTTCACTCGAGGAGGCGCTGACCAGCCCGGAGACGT GGGTGATCGGAGGCGGACAAGTCTATGCGCTGGCGCTGCCGTACGCGACCAGATGTGAGGTTACCGAGGT CGACATCGGCCTGCCGCGCGAAGCCGGTGACGCGCTGGCCCCCGTGCTGGACGAGACATGGCGGGGCGAG ACGGGGGAGTGGCGCTTCAGCCGGTCCGGGTTGCGGTACCGGTTGTACAGCTACCACCGCTCATGA Assume that you have been given a sample of the bacterial genomic DNA, restriction enzymes Ndel and BamHi, and a plasmid from Novagen called PET-28b....
please answer all 6 questions Question 27 3 pts TRBP is a protein important for the...
please answer all 6 questions
Question 27 3 pts TRBP is a protein important for the formation of the RISC complex. Which of the following would you expect in cells with null mutations in TRBP? o Reduced siRNA-mediated mRNA degradation o Increased miRNA-mediated translational repression o Increased deadenylase-mediated mRNA degradation o Reduced proteasome-mediated protein degradation D Question 28 3 pts A protein that binds to the 3' UTR of a VEGF mRNA and promotes deadenylation and uncapping is likely to:...
You isolate three E. coli mutants that are unable to synthesize histidine. You amplify the gene responsible for the phenotype from the wildtype (WT) and mutant strains (1, 2, and 3) using PCR and run the PCR products on an agarose gel. Based on the results, which mutant is likely to have an deletion in the gene of interest? (The top of the gel is where the DNA was loaded - the negatively charged end) Wt 1 2 3
Can you explain the answers below ?
Below is the same gene from page 1. Alternmative splicing of the primary RNA transcript will include either exon 4 or exon 5. Assume that mutants 1 and 5 are deletion mutants; mutant 2 is a frameshift mutation; and mutant 3, 4, and 6 are substitution mutations. PCR primers are designated with arrows and lowercase letters (a, b, c, d). mutant 4 mutant 61mutant 3 KR Kpnl Kpn ATG TAG TAA exon 1...
The most common mutation occuring in people with cystic fibrosis is the loss of a single amino acid (phenylalanine) at position 508 of the protein. This mutation O a. is a point mutation b. is an single nucleotide polymorphism c. is a deletion of 3 nucleotides d. in an insertion of a single nucleotide e. is a chromosomal rearrangement → Moving to another question will save this response. In Concience in 5 min you. For ence ne
please help me with both of these questions!!!!
3:33 PM Wed Mar 18 Enter answer... 5:06 Exit You are trying to amplify an eye color gene in Drosophila. Based on the DNA sequence for the gene, you design the following primers: Forward Primer: 5' GCATGCTGAG CCTAGTAACT 3 Reverse Primer: 5' CTTAAAGCTT ACTGGTCAAC 3' True or false? These are good primers to use in PCR. Hint: use the formula: Tm (in C) = 4(G+C) + 2(A + T) True False ormula:...
Answers are C,A. Please explain
the method to fix this kind of questions. Thanks
18. Shown below are the results of a series of coinfections of E. coli B using T4 rll- strains similar to those employed by Benzer in his deletion mapping experiment. Each strain contains a different deletion mutation. The ability to produce wild-type progeny phage is indicated by (+), (O) indicates no wild-type progeny. + + OC Which order of deletions is most consistent with this data?...
NEED HELP WITH THESE QUESTIONS. PLEASE ANSWER ALL AND EXPLAIN AS
WELL. THANKSSSSSSS
1. You want to clone a gene from a donor vector to a host vector. List the correct order of events in the process of cloning a. Perform ligation reaction of cloned gene and host vector. b. Perform double digestion of both donor and host vectors with the 2 restriction enzymes c. Examine donor and host vectors for restriction sites d. Purify cloned gene from donor vector...
very lost on this whole worksheet and i dont know if my
current answers are even right, please help i need this done by
today
Name: Cloning Worksheet chromosome qut 756bp pBiochem 5.983 bp ladder Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 BamHI (4233) (230) IT Enzyme recognition S ..GATATC. 51 EcoRV S .. . 3..CTATAC... 5 12 Pvull 89 BamHI Pett You are given the task to clone yfg into the plasmid pBioChem. Follow...
Note whether in the following strains of E. coli,
B-galactosidase will be:
(A) Abundant in both the presence and absence of lactose
(B) Abundant only in the absence of lactose
(C) Abundant only in the presence of lactose
(D) Abundant only in the presence of sucrose
(D) Absent in both the presence and absence of lactose
Can you answer and explain these please?
What is the correct gene order and which 2 genes are most closely linked? (4 Points) o+at...
1) Imagine that you are interested in studying the enzyme dihydrofolate reductase (DHFR) from Mycobacterium tuberculosis (this enzyme is a potential drug target). The amino acid sequence of the protein is: MTMVGCIWAQATSGVIGRGGDIPWRLPEDQAHFREITMGHTIVMGRRTWDSLPAKVRPLPGRRNWL SRQADFMASGAEWGSLEEALTSPETWVIGGGQVYALALPYATRCEVTEVDIGLPREAGDALAPVLD ETWRGETGEWRFSRSGLRYRLYSYHRS The DNA sequence within the M. tuberculosis genome that codes for the protein is: ATGACGATGGTGGGGCTGATCTGGGCTCAAGCGACATCGGGTGTCATCGGCCGCGGCGGCGACATCCCCT GGCGCTTGCCCGAGGACCAGGCGCATTTCCGGGAGATCACCATGGGGCACACGATCGTGATGGGCCGGCG CACATGGGATTCGCTGCCGGCTAAAGTCCGGCCGCTGCCCGGCCGGCGAAATGTCGTACTGAGCCGCCAA GCTGACTTTATGGCCAGCGGGGCTGAGGTTGTCGGTTCACTCGAGGAGGCGCTGACCAGCCCGGAGACGT GGGTGATCGGAGGCGGACAAGTCTATGCGCTGGCGCTGCCGTACGCGACCAGATGTGAGGTTACCGAGGT CGACATCGGCCTGCCGCGCGAAGCCGGTGACGCGCTGGCCCCCGTGCTGGACGAGACATGGCGGGGCGAG ACGGGGGAGTGGCGCTTCAGCCGGTCCGGGTTGCGGTACCGGTTGTACAGCTACCACCGCTCATGA Assume that you have been given a sample of the bacterial genomic DNA, restriction enzymes Ndel and BamHi, and a plasmid from Novagen called PET-28b....
please answer all 6 questions
Question 27 3 pts TRBP is a protein important for the formation of the RISC complex. Which of the following would you expect in cells with null mutations in TRBP? o Reduced siRNA-mediated mRNA degradation o Increased miRNA-mediated translational repression o Increased deadenylase-mediated mRNA degradation o Reduced proteasome-mediated protein degradation D Question 28 3 pts A protein that binds to the 3' UTR of a VEGF mRNA and promotes deadenylation and uncapping is likely to:...
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