Question

24. What is the chemical group responsible for making these amino acids acidic? 25. List the 3 amino acids that can be phosphorylated using single letter names 20. If you want to mutate Tyrosine (Y) to keep it from being negatively charged, but keep it as close keep it as close in shape as possible to tyrosine, which amino acid would you change it to Use the single letter name. being negatively charged but 27. If you want to mutate tyrosine (Y) to imitate the effect of phosphorylation, which amino acid would you change it to? Use the single letter name. Below is a sequence from a sheep PCR product. The template used was genomic DNA and the forward and reverse primers were designed to amplify partofthepriongene, which encodes the causative agento scrapie. Gisblack, Cisblue: Alsgreen: Tisred. The forward primarwasusedasa sequencingprimer. The Nattheleft arrow means that the computer can't decide which nucleotide is at that position in the sequence because there are two peaks (red=T and blues) that are the same height. The computer called the nucleotide A at the rightarrow, but it was wrong because there are two peaks at the same position of nearly the same height (green=A and black-G). IGFEL TOAG CAG GIC C A LEG CELA 28. Which strand of DNA would resultfrom sequencing with the forward primer ifthe top strand is the sense strand(translatable, same as mRNA sequence and the bottom strand is the non-sense strand? 29. Which strand of DNA would result from sequencing with the reverse primer if the top strandis the sense strand and the bottom strand is the non-sense strand? 30. Codons are indicated by vertical marks. What are the amino acids encoded by the codons at the First arrow (left) Secondarrow(right) 31. How would you label the: x-axis: y-axis 32. The arrowheads point to positions where there are two peaks. If the ratio of their height on the v-axis is! 1:1, how is this correlated with the genomic DNA template used and the prion gene alleles?

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Answer 1

Answer to question 24

Amino acids are organic compounds composed of an amino group and carboxyl group as substituents on alpha carbon atom.Basic strucure of amino acid contains an alpha carbon surrounded by hydrogen atom,carboxyl group,amino group and a variable R group.Number of amino and carboxyl group determines the acidic,basic and neutral character of amino acids.If number of carboxyl groups are greater than amino group the aminoacid is acidic in nature(eg,aspartic acid and glutamic acid, both having one amino group and two carboxyl group).