Homework Answers
3. 
4. CRISPR-Cas results in the expression of short crRNAs (CRISPR RNAs) which act as a guide in destruction of attacking or invading phages or plasmids DNA and RNA in bacteria and archaea.
5. tracrRNA is a trans-activating (acting from a different molecule) crRNA. This is a part of one of the CRISPR activation pathways.
It plays a role in the maturation of crRNA.
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please help answer these questions
3. What are the similarities, differences, advantages, and disadvantages of CRISPR-based...
4. The CRISPR-Cas9 system is an important new technique in molecular biology. What is the natural...
4. The CRISPR-Cas9 system is an important new technique in
molecular biology. What is the natural function of this system?
Describe how you would use this system to generate a null mutation
in another organism (i.e. explain Figure 6-43). How does it work?
What is the modification of the method that allows for correction
of a mutation (e.g. the mouse crystalline gene)? And lastly, what
are the problems with the CRISPR system?
FIGURE 6-43 Single-nucleotide mutations
can be introduced into...
Please help me answering these multiple questions of how the CRISPR system works, thank you! 1....
Please help me answering these multiple questions of how the CRISPR system works, thank you! 1. Match the CRISPR component with its function. (matching the upper case letters to the lower case letters) A). Cas9 B). gRNA (sometimes called sgRNA) C). Non-homologous End Joining (NHEJ) D). Homology-directed Repair (HDR) a). A short RNA molecule that guides a DNA cutting enzyme to a specific location in genomic DNA. b). A type of DNA repair mediated by a set of enzymes all...
Please have a different answer than the other post, and use layman's terms as well hhmi...
Please have a different answer than the other post, and use
layman's terms as well
hhmi Biolnteractive Using CRISPR to identify the Functions of Butterfly Genes Activity Student Handout This document is made available by the Howard Hughes Medical Institute. Using this document, you agree to use this document in accordance with the Terms of Use. INTRODUCTION Scientists have determined the complete DNA sequences of the genomes for many organisms, including humans. By analyzing patterns in those sequences, they can...
3) Antibodies can be membrane-bound or secreted, depending on whether a stretch of additional amino acids...
3) Antibodies can be membrane-bound or secreted, depending on whether a stretch of additional amino acids are present or not. What process do you think determines if these amino acids are present? postranslational covalent attachment of a membrane spanning domain to the antibody alternative RNA splicing phosphorylation polyadenylation ubiquitin addition 10) How does the Cas9 system target where it produces a double-strand break in the DNA? The Cas9 protein binds to a recombinase, allowing it to disable the gene of...
ASAP please .. Hey, I need help answering these questions, about the introduction of an article...
ASAP please ..
Hey, I need help answering these questions, about the introduction
of an article about HIV inhibition using adenovirus-delivered
CRISPR/CAS9. Thaanks!!
strand, resulting in double-stranded breaks (DSBs) that trigger cellular repai mechanisms. In eukaryotes, the DSBs are more commonly repaired by the mechanism ot error prone non ho mologous end joining (NHEJ), therefore generating sequence changes, for instance insertions and deletians (indels), around the DSBs. Owing to the simplicity of manipulation and versati ity, the CRISPR/Cas9 system has been...
please answer all the questions Question 8 0 / 1 pts Our understanding of RNA ...
please answer all the questions
Question 8
0 / 1 pts
Our understanding of RNA
was non-existent until 2000
started with the identification of a tRNA which suggested a
method of converting DNA to protein
began to identify that DNA-->protein--> RNA
stopped growing after it's original discovery in the 70s
IncorrectQuestion 10
0 / 1 pts
Enzymes allow for chemical reactions to occur in the cell that
may not naturally occur at the right place at...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...
A cell's genome is its blueprint for life. However, what is the bare minimum number of...
A cell's genome is its blueprint for life. However, what is the bare minimum number of genes needed to sustain a free-living cell? This is a question that microbiologists at the J. Craig Venter Institute (JCVI) have attempted to answer ever since they sequenced the genomes of several Mycoplasma species in the 1990s. Because Mycoplasma species are parasitic bacteria, their genomes are already reduced in size and hence provide an excellent foundation for creating a "minimal cell." However, little did...
please answer all the questions right. J. TIIS IS 18 Pelresh your memory on what you...
please answer all the questions right.
J. TIIS IS 18 Pelresh your memory on what you knew well and what you ne ork more on. It also is preparation for a review of all of the practice questions from unit 3, which is due Mon ay want to work on these quizzes together, or use this quiz to guide your studying and the old question revie onday to assess your studying. D l Question 1 0.2 pts (LO 15.7) How...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
4. The CRISPR-Cas9 system is an important new technique in
molecular biology. What is the natural function of this system?
Describe how you would use this system to generate a null mutation
in another organism (i.e. explain Figure 6-43). How does it work?
What is the modification of the method that allows for correction
of a mutation (e.g. the mouse crystalline gene)? And lastly, what
are the problems with the CRISPR system?
FIGURE 6-43 Single-nucleotide mutations
can be introduced into...
Please have a different answer than the other post, and use
layman's terms as well
hhmi Biolnteractive Using CRISPR to identify the Functions of Butterfly Genes Activity Student Handout This document is made available by the Howard Hughes Medical Institute. Using this document, you agree to use this document in accordance with the Terms of Use. INTRODUCTION Scientists have determined the complete DNA sequences of the genomes for many organisms, including humans. By analyzing patterns in those sequences, they can...
ASAP please ..
Hey, I need help answering these questions, about the introduction
of an article about HIV inhibition using adenovirus-delivered
CRISPR/CAS9. Thaanks!!
strand, resulting in double-stranded breaks (DSBs) that trigger cellular repai mechanisms. In eukaryotes, the DSBs are more commonly repaired by the mechanism ot error prone non ho mologous end joining (NHEJ), therefore generating sequence changes, for instance insertions and deletians (indels), around the DSBs. Owing to the simplicity of manipulation and versati ity, the CRISPR/Cas9 system has been...
please answer all the questions
Question 8
0 / 1 pts
Our understanding of RNA
was non-existent until 2000
started with the identification of a tRNA which suggested a
method of converting DNA to protein
began to identify that DNA-->protein--> RNA
stopped growing after it's original discovery in the 70s
IncorrectQuestion 10
0 / 1 pts
Enzymes allow for chemical reactions to occur in the cell that
may not naturally occur at the right place at...
please answer all the questions right.
J. TIIS IS 18 Pelresh your memory on what you knew well and what you ne ork more on. It also is preparation for a review of all of the practice questions from unit 3, which is due Mon ay want to work on these quizzes together, or use this quiz to guide your studying and the old question revie onday to assess your studying. D l Question 1 0.2 pts (LO 15.7) How...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
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