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Copy of In the CRISPR/Cas9 genome editing system, the job of the guide RNA is to......
While comparing a karyotype stained by FISH, you notice there is a terminal chromosomal deletion of...
While comparing a karyotype stained by FISH, you notice there is a terminal chromosomal deletion of the p arm of chromosome 5 which causes Cri du chat syndrome. Loss of a large chromosome segment like this can be the result of a which is most often caused by V If this damage had been fixed by this mutation would not have occurred While comparing a karyotype stained by FISH, you notice there is a terminal chromosomal deletion of the p...
You wish to edit a gene in a population of stem cells using CRISPR/Cas9. You design...
You wish to edit a gene in a population of stem cells using CRISPR/Cas9. You design a plasmid and transfect it into the cells. Your plasmid included the guide RNA sequence with promoter, the Cas9 gene inserted between an appropriate promoter and termination sequence, and the usual Origin of Replication and Resistance/Marker gene. However, instead of being edited, your target gene is silenced and its protein is not produced at all. What is the most likely explanation? The cells have...
You have designed a CRISPR/Cas9 plasmid and have successfully transfected it into human cells. However, when...
You have designed a CRISPR/Cas9 plasmid and have successfully transfected it into human cells. However, when you sequence the genome of these cells a week later, it appears that the cuts were made in the wrong location, silencing the wrong genes. This is most likely due to a problem with... the guide RNA O Cas9 the Shine-Dalgarno sequence on the plasmid O the Cas9 promoter on the plasmid the transfection procedure O
4. The CRISPR-Cas9 system is an important new technique in molecular biology. What is the natural...
4. The CRISPR-Cas9 system is an important new technique in
molecular biology. What is the natural function of this system?
Describe how you would use this system to generate a null mutation
in another organism (i.e. explain Figure 6-43). How does it work?
What is the modification of the method that allows for correction
of a mutation (e.g. the mouse crystalline gene)? And lastly, what
are the problems with the CRISPR system?
FIGURE 6-43 Single-nucleotide mutations
can be introduced into...
Which of the following statements is FALSE? In the context of CRISPR-Cas9 gene editing system, the...
Which of the following statements is FALSE? In the context of CRISPR-Cas9 gene editing system, the PAM sequence: a. A sequence adjacent to the target DNA sequence to be cleaved b. Is present on the non-complementary target DNA strand to the crRNA c. Has the sequence “5’-NGG-3’” where N is any nucleotide d. Stands for "protospacer adjacent motif" e. Is present in the bacterial genome
All of the following are required for genome editing with the CRISPR-Cas 9 system except: A....
All of the following are required for genome editing with the CRISPR-Cas 9 system except: A. Cas9 nuclease B. An sgRNA C. A repair mechanism for a double-strand DNA break (DSB) D. Invading DNA protospacers E. A PAM sequence
<Problem Set 9a Question 4 4 of 5 > Part A - TUA: Uverview UUR HIDRL...
<Problem Set 9a Question 4 4 of 5 > Part A - TUA: Uverview UUR HIDRL yur g pruun When scientists use CRISPR-Cas9 to make specific cuts to a genome, they have a goal they're trying to achieve. Ohen, they either want to modify the protein that a particular gene encodes, or they want to block production of a particular protein. Depending on the goal, they customize the SANA they insert into the cell. For the CRISPR-Cas9 system to modify...
4. What is the function of each component of the CRISPR-Cas9 system? (1.5 pt) PAM site:...
4. What is the function of each component of the CRISPR-Cas9 system? (1.5 pt) PAM site: Guide RNA: Cas9: 5a. In the DNA below, the "I" line indicates where there is a mutation in a gene. Design a gRNA to find this mutation. Your gRNA will be 8 bases long and will not include the PAM sequence. Keep the following in mind: [2 pts total] i. Scan the top strand left to right and find the PAM site. ii. When...
omework Part D-Question 94 CRISPR-Cas9 can be used to 1:59 PM disable genes O fix disease...
omework Part D-Question 94 CRISPR-Cas9 can be used to 1:59 PM disable genes O fix disease genes O add new genes O remave existing genes rk - watch two vidoo change gene sequences Submit My Answers Give Up Incorrect; Try Again; 5 attempts remaining ology Part E·Question #5 How does the CRISPR-Cas9 system speofically target DNA sequences n. All rights reserved licy Permissions O It make specific recombinant DNA sequences that match the target O It degrades the ONA from...
Question 7 (1 point) CRISPR/Cas9 is often used to make mutations in zebrafish. Which statement is...
Question 7 (1 point) CRISPR/Cas9 is often used to make mutations in zebrafish. Which statement is most correct? Capping the sgRNA is essential for its stability. The Cas9 mRNA is is used to target the native Cas9 protein to the locus to be mutated. the sgRNA uses DICER to cut up a the twenty nucleotide target region of the DNA, starting at the 3-prime end. O neomycin is used to select the cells that inherit the Cas9 insertion. O the...
While comparing a karyotype stained by FISH, you notice there is a terminal chromosomal deletion of the p arm of chromosome 5 which causes Cri du chat syndrome. Loss of a large chromosome segment like this can be the result of a which is most often caused by V If this damage had been fixed by this mutation would not have occurred While comparing a karyotype stained by FISH, you notice there is a terminal chromosomal deletion of the p...
You wish to edit a gene in a population of stem cells using CRISPR/Cas9. You design a plasmid and transfect it into the cells. Your plasmid included the guide RNA sequence with promoter, the Cas9 gene inserted between an appropriate promoter and termination sequence, and the usual Origin of Replication and Resistance/Marker gene. However, instead of being edited, your target gene is silenced and its protein is not produced at all. What is the most likely explanation? The cells have...
You have designed a CRISPR/Cas9 plasmid and have successfully transfected it into human cells. However, when you sequence the genome of these cells a week later, it appears that the cuts were made in the wrong location, silencing the wrong genes. This is most likely due to a problem with... the guide RNA O Cas9 the Shine-Dalgarno sequence on the plasmid O the Cas9 promoter on the plasmid the transfection procedure O
4. The CRISPR-Cas9 system is an important new technique in
molecular biology. What is the natural function of this system?
Describe how you would use this system to generate a null mutation
in another organism (i.e. explain Figure 6-43). How does it work?
What is the modification of the method that allows for correction
of a mutation (e.g. the mouse crystalline gene)? And lastly, what
are the problems with the CRISPR system?
FIGURE 6-43 Single-nucleotide mutations
can be introduced into...
<Problem Set 9a Question 4 4 of 5 > Part A - TUA: Uverview UUR HIDRL yur g pruun When scientists use CRISPR-Cas9 to make specific cuts to a genome, they have a goal they're trying to achieve. Ohen, they either want to modify the protein that a particular gene encodes, or they want to block production of a particular protein. Depending on the goal, they customize the SANA they insert into the cell. For the CRISPR-Cas9 system to modify...
4. What is the function of each component of the CRISPR-Cas9 system? (1.5 pt) PAM site: Guide RNA: Cas9: 5a. In the DNA below, the "I" line indicates where there is a mutation in a gene. Design a gRNA to find this mutation. Your gRNA will be 8 bases long and will not include the PAM sequence. Keep the following in mind: [2 pts total] i. Scan the top strand left to right and find the PAM site. ii. When...
omework Part D-Question 94 CRISPR-Cas9 can be used to 1:59 PM disable genes O fix disease genes O add new genes O remave existing genes rk - watch two vidoo change gene sequences Submit My Answers Give Up Incorrect; Try Again; 5 attempts remaining ology Part E·Question #5 How does the CRISPR-Cas9 system speofically target DNA sequences n. All rights reserved licy Permissions O It make specific recombinant DNA sequences that match the target O It degrades the ONA from...
Question 7 (1 point) CRISPR/Cas9 is often used to make mutations in zebrafish. Which statement is most correct? Capping the sgRNA is essential for its stability. The Cas9 mRNA is is used to target the native Cas9 protein to the locus to be mutated. the sgRNA uses DICER to cut up a the twenty nucleotide target region of the DNA, starting at the 3-prime end. O neomycin is used to select the cells that inherit the Cas9 insertion. O the...
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