Homework Answers
Ans). the reason why the targeted gene is silenced ans its protein is not produced at all because as the the gene expression changes the RNA polymeraseis ineffective in producing the reqiued protein result in protein is not produced. Hence the cells have a defective RNA polymerase.
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You wish to edit a gene in a population of stem cells using CRISPR/Cas9. You design...
You have designed a CRISPR/Cas9 plasmid and have successfully transfected it into human cells. However, when...
You have designed a CRISPR/Cas9 plasmid and have successfully transfected it into human cells. However, when you sequence the genome of these cells a week later, it appears that the cuts were made in the wrong location, silencing the wrong genes. This is most likely due to a problem with... the guide RNA O Cas9 the Shine-Dalgarno sequence on the plasmid O the Cas9 promoter on the plasmid the transfection procedure O
7.. In the controlled termination method of DNA sequencing, reading the gel from _____ gives the...
7.. In the controlled termination method of DNA sequencing, reading the gel from _____ gives the sequence in the _____ direction; _____ fragments that were terminated _____ in polymerization move faster down the gel a. bottom to top; 5′ to 3′; shorter; early b. top to bottom; 5′ to 3′; longer; early c. bottom to top; 5′ to 3′; longer; later d. top to bottom; 5′ to 3′; shorter; early e. bottom to top; 3′ to 5′; shorter; early 8....
Two CRISPR experiments (A and B) have been conducted in yeast cells to understand the effect...
Two CRISPR experiments (A and B) have been conducted in
yeast cells to understand the effect of inducible promoters. The
yeast cells used in both experiments contain 2 plasmids: one
plasmid that has the Cas9 gene and one plasmid that has the CAN1.Y
gRNA sequence. However, the two experiments differ in the promoter
used in one of the plasmid.
The graph below shows the colony count from the
canavanine selection plates (YNB + met + his + canavanine) that use...
1. what is CRISPR gene editing? 2. what enzyme is used to cut DNA? 3.Why do...
1. what is CRISPR gene editing? 2. what enzyme is used to cut DNA? 3.Why do bacteria use CRISPR? 4.How is the enzyme targeted to a specific DNA sequence? 5.what need to be done in order to make sure the proper human protein is made in bacteria cells? 6.How can you check for proper orientation of the inserted DNA in the plasmid? 7. Why are iPSCs useful for studying hereditary neuronal diseases such as Alzheimer's. 8. How are pig cells...
<Problem Set 9a Question 4 4 of 5 > Part A - TUA: Uverview UUR HIDRL...
<Problem Set 9a Question 4 4 of 5 > Part A - TUA: Uverview UUR HIDRL yur g pruun When scientists use CRISPR-Cas9 to make specific cuts to a genome, they have a goal they're trying to achieve. Ohen, they either want to modify the protein that a particular gene encodes, or they want to block production of a particular protein. Depending on the goal, they customize the SANA they insert into the cell. For the CRISPR-Cas9 system to modify...
What would be the composition of the canavanine selection plates (see Lab 9) if we did...
What would be the composition of the canavanine selection plates
(see Lab 9) if we did not chose to select for the presence of the
gRNA plasmid?
Plate counting Last week you transformed the PMD2 plasmid that contains the ORNA sequence. The yeast cells were selected on a YNB + histidine + methionine plate in order to make sure that only the cells that contain the plasmid grow and give a colony. Simply count the number of colonies that grew...
23. You want to edit the vinculin gene of a stable human cell line using pCAS-Guide...
23. You want to edit the vinculin gene of a stable human cell line using pCAS-Guide vector. Which of the following you do not have to have to do? A. Clone part of the vinculin gene in the pCAS-Guide vector B. Transfect the recombinant pCAS-Guide vector to the cell line C. Inject a small amount of cas 9 protein in the cells D. Insert a marker gene inside the cell The siRNA, miRNA and piRNA of eukaryotes 24. An antisense...
You are studying a human protein, XYZ, that localizes to the nucleus in human cells. You...
You are studying a human protein, XYZ, that localizes to the nucleus in human cells. You want to see if it will also localize to the nucleus in mouse cells. To do this, you clone a translational fusion of the jellyfish fluorescent protein, called GFP, to XYZ and put it into a plasmid. This means that XYZ and GFP are now translated as a single protein. Since XYZ is now fused to GFP, you can simply image GFP to see...
1) You have two human liver cells (A and B) and you hypothesize that the insulin...
1) You have two human liver cells (A and B) and you hypothesize that the insulin receptor gene in Cell A has a mutation in exon 1 and Cell B contains the wild type sequence. You extract genomic DNA from each of the cells. Of the following, what would be the most efficient (quick, precise and relatively cheap) way to test your hypothesis. a. Isolate protein from both cells, purify the insulin receptor, and determine the amino acid content. b. Sequence the...
please answer All the multiple choice questions in the pic (all pics) i dont need a...
please answer All the multiple choice questions in the pic (all
pics) i dont need a explantion .
22 Using a bacteriophage to pass DNA rom bacterium to another O A) Transduction O B) Transformation C) Translocation O D) Translation 23. What research did Rosalind Franklin contribute to the elucidation of the double helix structure of DNA? O O O A) Principles of base pairing B) Biochemical data C) Bacterial transformation data D) X ray crystallography A segment of DNA...
You have designed a CRISPR/Cas9 plasmid and have successfully transfected it into human cells. However, when you sequence the genome of these cells a week later, it appears that the cuts were made in the wrong location, silencing the wrong genes. This is most likely due to a problem with... the guide RNA O Cas9 the Shine-Dalgarno sequence on the plasmid O the Cas9 promoter on the plasmid the transfection procedure O
Two CRISPR experiments (A and B) have been conducted in
yeast cells to understand the effect of inducible promoters. The
yeast cells used in both experiments contain 2 plasmids: one
plasmid that has the Cas9 gene and one plasmid that has the CAN1.Y
gRNA sequence. However, the two experiments differ in the promoter
used in one of the plasmid.
The graph below shows the colony count from the
canavanine selection plates (YNB + met + his + canavanine) that use...
<Problem Set 9a Question 4 4 of 5 > Part A - TUA: Uverview UUR HIDRL yur g pruun When scientists use CRISPR-Cas9 to make specific cuts to a genome, they have a goal they're trying to achieve. Ohen, they either want to modify the protein that a particular gene encodes, or they want to block production of a particular protein. Depending on the goal, they customize the SANA they insert into the cell. For the CRISPR-Cas9 system to modify...
What would be the composition of the canavanine selection plates
(see Lab 9) if we did not chose to select for the presence of the
gRNA plasmid?
Plate counting Last week you transformed the PMD2 plasmid that contains the ORNA sequence. The yeast cells were selected on a YNB + histidine + methionine plate in order to make sure that only the cells that contain the plasmid grow and give a colony. Simply count the number of colonies that grew...
You are studying a human protein, XYZ, that localizes to the nucleus in human cells. You want to see if it will also localize to the nucleus in mouse cells. To do this, you clone a translational fusion of the jellyfish fluorescent protein, called GFP, to XYZ and put it into a plasmid. This means that XYZ and GFP are now translated as a single protein. Since XYZ is now fused to GFP, you can simply image GFP to see...
please answer All the multiple choice questions in the pic (all
pics) i dont need a explantion .
22 Using a bacteriophage to pass DNA rom bacterium to another O A) Transduction O B) Transformation C) Translocation O D) Translation 23. What research did Rosalind Franklin contribute to the elucidation of the double helix structure of DNA? O O O A) Principles of base pairing B) Biochemical data C) Bacterial transformation data D) X ray crystallography A segment of DNA...
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