Which of the following statements is FALSE? In the context of CRISPR-Cas9 gene editing system, the...
Which of the following statements is FALSE? In the context of CRISPR-Cas9 gene editing system, the PAM sequence:
a. A sequence adjacent to the target DNA sequence to be cleaved
b. Is present on the non-complementary target DNA strand to the crRNA
c. Has the sequence “5’-NGG-3’” where N is any nucleotide
d. Stands for "protospacer adjacent motif"
e. Is present in the bacterial genome
Homework Answers
e. Is present in the bacterial genome
The CRISPR-Cas9 gene editing system is a bacterial immune defence system. PAM sequences, when present adjacent to the target sequence, promote the cleavage of the complementary DNA strand by Cas9 after crRNA (or guide RNA in gene editing) recognizes the target sequence. Bacterial genomes do not have PAM sequences to avoid self-targeting by the CRISPR-Cas9 system.
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Which of the following statements is FALSE? In the context of
CRISPR-Cas9 gene editing system, the...
4. The CRISPR-Cas9 system is an important new technique in molecular biology. What is the natural...
4. The CRISPR-Cas9 system is an important new technique in
molecular biology. What is the natural function of this system?
Describe how you would use this system to generate a null mutation
in another organism (i.e. explain Figure 6-43). How does it work?
What is the modification of the method that allows for correction
of a mutation (e.g. the mouse crystalline gene)? And lastly, what
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Copy of In the CRISPR/Cas9 genome editing system, the job of the guide RNA is to......
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omework Part D-Question 94 CRISPR-Cas9 can be used to 1:59 PM disable genes O fix disease...
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4. The CRISPR-Cas9 system is an important new technique in
molecular biology. What is the natural function of this system?
Describe how you would use this system to generate a null mutation
in another organism (i.e. explain Figure 6-43). How does it work?
What is the modification of the method that allows for correction
of a mutation (e.g. the mouse crystalline gene)? And lastly, what
are the problems with the CRISPR system?
FIGURE 6-43 Single-nucleotide mutations
can be introduced into...
Copy of In the CRISPR/Cas9 genome editing system, the job of the guide RNA is to... o cut the target sequence The job of the guide RNA is not fully known yet. o identify the correct sequence to be cut o help the plasmid enter the cells hide the plasmid from the immune system
4. What is the function of each component of the CRISPR-Cas9 system? (1.5 pt) PAM site: Guide RNA: Cas9: 5a. In the DNA below, the "I" line indicates where there is a mutation in a gene. Design a gRNA to find this mutation. Your gRNA will be 8 bases long and will not include the PAM sequence. Keep the following in mind: [2 pts total] i. Scan the top strand left to right and find the PAM site. ii. When...
You wish to edit a gene in a population of stem cells using CRISPR/Cas9. You design a plasmid and transfect it into the cells. Your plasmid included the guide RNA sequence with promoter, the Cas9 gene inserted between an appropriate promoter and termination sequence, and the usual Origin of Replication and Resistance/Marker gene. However, instead of being edited, your target gene is silenced and its protein is not produced at all. What is the most likely explanation? The cells have...
please help answer these questions
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