Question

Question 7 (1 point) CRISPR/Cas9 is often used to make mutations in zebrafish. Which statement is most correct? Capping the sgRNA is essential for its stability. The Cas9 mRNA is is used to target the native Cas9 protein to the locus to be mutated. the sgRNA uses DICER to cut up a the twenty nucleotide target region of the DNA, starting at the 3-prime end. O neomycin is used to select the cells that inherit the Cas9 insertion. O the SgRNA targets Cas9 to the locus to be mutated. Cas9 facilitates homologous recombination of the sgRNA into the target locus, thus disrupting it. OsgRNA targets the RNA Pol and trx stops in the target gene. To knockdown the gene, sgRNAs targets the promoter region of the target gene. sgRNA makes a double stranded break in the target gene and the Cas9 protein repairs the DNA by ligation, making mistakes in the process.

Answer 1

Answer 7:) First option

Single-guide RNA (sgRNA) is quite unstable because of its short size and therefore, the 5’capping and 3’ polyadenylated tail becomes necessary to increase its efficiency and stability. The sgRNA is used to direct the Cas9 protein.

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