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CRISPR-Cas9 provides an unprecedented means of modifying the genome of almost any organism. Which of the...

CRISPR-Cas9 provides an unprecedented means of modifying the genome of almost any organism. Which of the following component(s) of this system provide(s) the specificity of the endonucleolytic cleavage?

the sgRNA

the PAM sequence

Cas9

a and b

a, b and c
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Answer #1

a, b and c

CRISPR/Cas9 gene targeting requires a custom single guide RNA (sgRNA) that contains a targeting sequence (crRNA sequence) and a Cas9 nuclease-recruiting sequence (tracrRNA). The crRNA region (shown in red below) is a 20-nucleotide sequence that is homologous to a region in your gene of interest and will direct Cas9 nuclease activity

Protospacer adjacent motif (PAM) is a 2-6 base pair DNA sequence immediately following the DNA sequence targeted by the Cas9 nuclease in the CRISPR

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