Question

Prior to gene therapy with the agile allele (A), a male rat was homozygous (aa) for the autosomal recessive trait, awkwardness. Following the completion of the gene therapy, this same rat was test- crossed with a female rat of the appropriate genotype. What genotype should the female rat have in order for her to be used in a testcross? The offspring produced in the testcross were as follows: Assuming that the lest cross was performed correctly, what type of gene therapy must have been performed on the homozygous, awkward male rat? Somatic cell gene therapy? Germline gene therapy? Please explain. You have another, different, mutant rat: one that suffers from a lack of cilia in the airway (of the respiratory tract). Suggest a method of gene therapy that could be used introduce cilia into this rat's airway. Specifically, describe how the gene responsible for cilia production will be introduced and why you chose this specific method. Is it likely that the problem will be permanently "fixed", or would repeated treatments be necessary? Why? Cite a legitimate reason, preferably one that is based upon the mechanism by which the ''good' gene is introduced into the cells.

Answer 1

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