Question

Due date-September 6, 2017 Bi042000-Problem Set #1 Name: 2. budy identified a new virus OHawk virus) that has an RNA genome and is causing the death of red tailed hawks in Manhattan. Upon further analysis of the Hawk virus Ife cycle, she found that the virus must synthesize DNA in order to replicate in Vero cells, which are susceptible and permissive for Hanwk virus a. Using your knowledge of the Baltimore classification system of viruses, draw a flow chart showing the steps that are required to synthesize mRNA from the Hawk virus genome. Be sure to indicate whether the DNA or RNA is single-stranded or double-stranded and whether the DNA or RNA is the positive or negative strand. (2 points) b. Judy sequences the Hawk capsid open reading frame (ORF). She purified genomic DNA from Vero cells that were infected with Hawk virus. She used PCR primers to amplify the Hawk genome and subsequently used a third primer to sequence the Hawk capsid ORF, which is shown below 5-ATG ACG STA TAC GGT CGA TAA-3 Using the capsid ORF sequence, write the sequence of the DNA that is produced immediately after the Hwk virus indects the Vero cells. Be sure to indicate the polarity of the DNA. 2 points More questions on the beckside

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Answer 1

eDNA ↓ DNA A- RNA P チ

/b