Question

ssessable tutorial que 03/2018 The conversion of corn starch to dextrose using the enzyme glucoamylase was studied in a batch reactor at 60°C. The initial concentration of corn starch was 25 gl. The dosage of glucoamylase, Eo, was 11.6 mgL-. Figure 1, below, shows a Lineweaver-Burk plot for results obtained for the starch to dextrose enzymatic process. 0.13 0.12 0.11 0.10 Slope of plot 1.1 hr y-intercept of plot-0.033 hr L/g 0.09 0.08 0.07 0.04 0.05 0.06 0.07 0.08 0.09 1/C, (LUg) Figure 1. Lineweaver-Burk plot for corn to dextrose with glucoamylase enzyme i) The slope of the plot is 1.1 hr and the 'y-intercept' (i.e. the value of 1/(-rs) when 1/Cs O) is 0.033 ii) Determine the time the batch reactor must be operated for the starch concentration to decline to hr L/g. Determine the Michaelis-Menten parameters Vmax and KM 5 gL. (Assume the reaction conditions of temperature, pressure and enzyme concentration are the same as that used for generating the Lineweaver-Burk plot (above)) iii) Repeat problem (ii) for the case where the enzyme concentration is 25.9 mgL

Answer 1