Question

5) The reaction, 2A + B P (P is the product). proceeds via a rapid pre-equilibrium (with equilibrium constant, K) followed by a slow rate determining step (with rate constant, kz) as shown below: 2A к = Az (fast pre-equilibrium) A2+B Products (slow) Develop an expression for the rate of formation of products as a function of [A], [B], K, and kz 6) The reaction, A+B+C - P(P is the product) proceeds by the following mechanism. k, A+B=0 I+CP "T" is an intermediate present in steady-state concentration. Use the steady-state approximation on U to develop an expression for the rate of formation of P as a function of [A], [B], [C],k, ky, and k. 7) The enzyme-catalyzed conversion of a substrate at 25°C has a Michaelis constant of 0.045 molar. The rate of the reaction is 1.15 mmolars when the substrate concentration is 0.110 molar. What is the maximum velocity of the enzymolysis? 8) The hydrolysis of the substrate, N-glutaryl-L- phenylalanine-p-nitroanilide (GPNA), to p-nitroaniline and N-glutaryl-L-phenylanine is catalyzed by - chymotrypsin. When [E]0=4.0 x 10M, it is found that V=3.7 x 10 M/s when [S]=2.5 x 10-M and v=9.8 x 10 M/s when [S]=1.0x10 M. Determine the slope and the intercept of the Lineweaver-Burk plot and, from these, K. Vm, and k for this reaction. 9) An enzyme has the Michaelis-Menten parameters, KM=2.18x10-5 M and Vm=53 mM/min. For [S[=7.5x10M and [1]=4.8 x10M, and KI=1.7x10* M.

Answer 1

As per HomeworkLib rule I'm answering ist question. The objective is to develop an expression for the rate of formation of products to the following. Ans Given that, . ZA A₂ (1) K=0 . [Ar] . [Az Az + B p (product). The rate of formation of product CP)decke [A21 (03-2) ((x and y are the order of reaction with respect to de and B respectively.). from equation , [Ar] = K[A². putting the value of [Ar] into equation we get, df - keşk[A]*}" CBy: Ke.x* [azza CB0% ... The required expression for the formation of product is di - keiket (Ajex (BJP. dt dt