s- (3 pts) The flowing rates have been an enzyme- catalyzed reaction at various substrate concentrations:...
Initial rates of an enzyme-catalyzed reaction for various substrate concentrations are listed in the table below. From this data determine Km and Vm S(M)v (HM/min) 4.1x103 9.5x104 5.2x104 1.03x104 4.9x10-5 1.06x10 5.1x10-6 173 125 106 80 67 43
10.11 The initial rates at various substra tions for an enzyme-catalyzed reaction are ious substrate concentra ed reaction are as follows: Vo/10-6 M min-1 38.0 53.4 S/M 2.5 x 10-5 4.00 x 10-5 6.00 x 10-5 8.00 x 10-5 16.0 x 10-5 20.0 x 10-5 68.6 80.0 106.8 114.0 (a) Does this reaction follow Michaelis-Menten kinet- ics? (b) Calculate the value of V max of the reaction. (c) Calculate the K value of the
3.33 3. Suppose that the following data are obtained for an enzyme-catalyzed reaction: [S(mm) V (mmol ml-Imin-1) 0.1 0.2 5.00 7.14 8.0 1.0 8.33 9.09 (a) From a double-reciprocal plot of the data, determine Km and Vmax. (b) Assuming that the enzyme present in the system had a concentration of 10-6 M, calculate its turnover number 0.8 2.0