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10.11 The initial rates at various substra tions for an enzyme-catalyzed reaction are ious substrate concentra ed reaction ar

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Q10.11 (a) For the given data, the Lineweaver Burk plot gives a straight line. Hence, this reaction follows Michaelis-Menten kinetics.

(b) The equation of straight line is : y = 0.5013x + 6234.3

slope = 0.5013 min

intercept = 6234.3 min/M

Vmax = 1/intercept

Vmax = 1/(6234.3 min/M)

Vmax = 1.604 x 10-4 M/min

Vmax = 160.4 x 10-6 M/min

(c) Km = (slope) / (intercept)

Km = (0.5013 min) / (6234.3 min/M)

Km = 8.04 x 10-5 M

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