by forming an ensyme- Dubatrate 2. How do we use absorbance to monitor the progress of...
Why do we use absorbance to monitor the reaction rate? (in an experiment with crystal violet). Is the correct answer a, b, c, or d? a. Crystal violet is poisonous and cannot be titrated b. Spectrophotometery is an inexpensive method c. Crystal violet and basic phenolphthalein are colored solutions d. All of the above
Wanting to monitor the reaction progress you decide to use normal phase thin-layer chromatography, but do not know what the best conditions are to use. If you had the standards for all potential products and your starting materials, outline how would you go about determining the best TLC conditions for monitoring the reaction progress. Provide drawings of an ideal TLC plate with the number of theoretical products you would expect and their relative positions. How do you know when the...
Given the reaction below, consider how you could use IR spectroscopy to monitor the progress of the reaction. Select the correct instruction(s).
Why do you think it is important for an organization to continually monitor their progress? How would you make adjustments when you notice something isn’t working? What impact could it have on the organization if you don’t make the changes immediately?
Describe how IR spectroscopy might be used to monitor the progress of the following reaction: H2Cr04 OH OH H20, H2S04 1-butanol butanoic acid
TLC is often used to monitor the progress of chemical reactions. For example for the reaction A + B -----> C, how could you determine when the reaction is done? Explain thoroughly.
When using a spectrometer to measure the absorbance as a function of concentration, why do we have to plot our calibration curve at the maximum wavelength (lambda max)? I know it's to get the highest point of absorbance and therefore ensure high sensitivity, but how does it affect my answers on the calibration curve? what if I didn't use lambda max?
How do I use Beer's law to convert all my absorbance data into concentration data in excel (and also by hand)? There are three different temperatures in excel. Let's say 10 Celcius, 20 C and 30. They all have the same concentration of the solution used which was 3.0*10^-5 and the length was 2 cm. How do I do this on excel and then generate a data vs. time graph? I also need to create graphs with Ln (temperatures) and...
Explain how Thin Layer Chromatograpy (TLC) was used to monitor the progress of the second step of the diantilis synthesis ( the conversion of the benzyl alcohol into the diantilis product). Illustrate your answer by drawing representative TLC plates.
Table 1. Concentrations of [Fe(Phen),1?, M, in water and UV- vis absorbance values recorded at 2 -505 nm. Concentration of [Fe(Phen)/, M Absorbance 9.500 x 10 0.999 4.250 x 10 0.480 0.219 2.125 10% 1.063 x 10 0.104 0.000 0.003 Table 2. UV-vis absorbance values of [Fe(phen)]* held at 35 °C after mixing with H SO, and recorded at 5 minute intervals (in water at max = 505 nm). Time (minutes) Absorbance 0.997 0.935 0.877 0.823 0.772 0.724 0.680 0.638...