See image
please show full solution, (Indicate your answers to two digits after the decimal)
Ingredient | Volume (uL) |
EcoRI digested plamsid A | 1.5 |
50ng EcoRI digested plasmid B | 0.5 |
5X ligation buffer | 10 |
T4 DNA ligase | 1 |
Water | 37 |
Plasmid A stock concentartion is 100ng/ul we need 150ng so 1.5ul
Plasmid B stock concentration is 100ng/ul we need 50ng so 0.5ul
See image please show full solution, (Indicate your answers to two digits after the decimal) 8....