Question

Question 1 (1 point) v Saved A recombinant protein corresponding to 52 repeats of the peptide sequence YSPTSPS was covalently linked to agarose beads to make an affinity column. The beads were incubated with a nuclear extract and the protein:beads became phosphorylated. More nuclear extract was run over the column repeatedly and then the beads were washed extensively. The bound proteins were eluted from the column and when they were separated on a SDS-PAGE gel many bands appeared. What cellular process(es) might these proteins be involved in? O splicing O polyadenylation O TEIIH a and b only Ob and c only

Answer 1

Answer: Option D is correct
Explanation:
The RNA polymerase II CTD contains ~52 repeats of the sequence YSPTSPS.
This CTD interacts with several factors involved in post-transcriptional modifications such as splicing factors and poly-adenylation factors.
The RNA pol II CTD is reported to coordinate transcriptional and splicing.
Several proteins involved in polyadenylation such as CPSF and CstF are shown to interact with CTD.