The GTPase Dynamin is involved in which step of vesicular A) Binding of cargo molecules to...
1) how does the timing and location of Arf1-GTP hydrolysis contribute the transport of COPI coated vesicles? 2) Consider cargo proteins that are destined for secretion to the extracellular space. For each of the following pairs of mutations or conditions, predict where the cargo will end up (ex. in the cytoplasm, a specific organelle, transport vesicles, multiple places, extracellular space) for: (i) & (ii) Each individual condition; & (iii) The combination of both. A.(i) Normal Sar1 is mutated so that...
2. Tethering of a transport vesicle to a target membrane involves all of the following players EXCEPT: Group of answer choices A. Rab effector proteins B. SNAREs C. Rab D. GTP 3. Match the following proteins with a brief description of their role in vesicle trafficking: A. Coat Protein B. required for constricting and closing vesicles C. mediate the selective recruitment of both membrane and soluble cargo molecules into the vesicle D. aid vesicle targeting and mediate membrane fusion E....
Detail methods of vesicular transport. Include in your discussion: clathrin, COPI, COPII, triskelion, cargo receptor, adaptor protein, BAR domain proteins, dynamin, SNARE proteins, Rab-GTP, Rab effector. Describe the role of phosphoinositides, listing the compartment specificity of various PIPs.
Please answer all questions 2 After isolating the rough endoplasmic reticulum from the rest of the cytoplasm, you purify the RNAS attached to it. Which of the following proteins do you expect the RNA from the rough endoplasmic reticulum to encode? (a) (c) soluble secreted proteins plasma membrane proteins ER membrane proteins all of the above (b) (d) -13 In which cellular location would you expect to find ribosomes translating MRNAS that encode ribosomal proteins? (a) (c) the nucleus in...
The signal sequence found in proteins that are sorted to the matrix of the mitochondrion is? A. an N-terminal amphiphilic α-helix that is cleaved from the protein by a signal peptidase in the matrix of the mitochondrion B. an N-terminal amphiphilic α-helix that is not removed from the protein C. an N-terminal sequence of at least eight hydrophobic residues that is not removed from the protein D. five adjacent (+)-charged amino acids found anywhere within the protein sequence; this signal...
1. The formation of a stable ternary complex involving Ran GTPase, a nuclear transport receptor, and a cargo protein occurs in ... A. both nuclear import and export. B. nuclear import , but not export. C. nuclear export, but not import. D. neither nuclear import nor export. 2. Compared to the cytosol, which of the following is generally true about the lumen of the endoplasmic reticulum in our cells? It has a much higher calcium ion concentration. It has a...
please answer both Which of the following is NOT TRUE regarding both M6P and KDEL receptors? Select one: a. They help transport cargo to their proper destinations b. They bind to cargo proteins in the Golgi apparatus. c. They are transported by vesicle budding and fusion, d. They move back-and-forth between different organelles. O e. They release soluble cargo proteins at lower pH. Which of these is NOT a function of the Signal Recognition Particle (SRP) involved in transport of...
Help please. Thanks 14. Adenylyl cyclase produces the intracellular signal: a) Kinase b) Phosphatase c) Cyclic AMP d) AMP 15. When adrenalin is produced PKA is turned off a) b) PKA is turned on c) Has no effect on PKA d) None of the above 16. All proteins with a signal sequence enter the ER 17. The sorting signal, Nuclear Localization Sequence, all proteins in the cytosol to enter the 18. In order for proteins to enter the mitochondria, they...
on the type of cell resulting in new gene e enters the nucleus and activates specifie a step in the regulated the coat b) dynamin binds to the cytosolic e) the adaptor proteins called Sec2seSarl to relea SNAREs 14. The ed assembly of the coat found on a CoPll vesicle is surface of the donor membrane that will form the vesicle d) a Sarl-GEF in the donor membrane ca e) specitie -SNARES in the donor mem that 15. T membrane...
Consider cargo proteins that are destined for secretion to the extracellular space. Please predict where the cargo will end up (ex. in the cytoplasm, a specific organelle, transport vesicles, multiple places, extracellular space) for: (i) & (ii) Each individual condition; & (iii) The combination of both conditions. A. (i) Normal Sar1 is mutated so it binds tightly to GDP and cannot exchange it for GTP. (This leads to Sar1-GDP being the only form of the protein in the cell.) (ii)...