Question

Rank the following subcellular locations from most (4) to least (1) acidic: ER [Choose] Golgi [Choose] lysosome [Choose] Mitochondrial matrix [Choose]

2. Tethering of a transport vesicle to a target membrane involves all of the following players EXCEPT:

Group of answer choices

A. Rab effector proteins

B. SNAREs

C. Rab

D. GTP

3. Match the following proteins with a brief description of their role in vesicle trafficking:

A. Coat Protein

B. required for constricting and closing vesicles

C. mediate the selective recruitment of both membrane and soluble cargo molecules into the vesicle

D. aid vesicle targeting and mediate membrane fusion

E. GTPase that guides transport vesicle to their target

1. Rab

2. Dynamin

3. Clathrin

4. Adaptor Proteins

5. SNAREs

Answer 1

Most to least acidic- lysosomes- 4.5, mitochondrial matrix- 8.0, ER- 9.0, Golgi- 11-13

2. D. GTP

3. A. Coat protein- 3. clatharin- performs in shaping rounded vesicles in cytoplasm for intracellular trafficking. The clatharin coated vesicles target selectively at cell membrane, endosomal compartments etc.. Clatharin mediated endocytosis is a vesicular transport which facilitates internalization and recycling of the receptors which include signal transduction pathways, synaptic vesicle formation etc..

B. Rab- vesicle movement across actin and tubulin networks by which cell surface proteins are trafficked from golgi to plasma membrane and are recycled back.

C. adaptor proteins- binds to clatharin and membrane bound cargo receptors. they mediate membrane trafficking along endocytic and secretory transport pathways, they usually recognize and concentrate cargo proteins into vesicular carriers.

D. SNARE's- ER localized vesicle snare proteins sec-22 plays a major role in anterograde and retrograde transport by generating efficient membrane fusion. In anterograde transport cargo vesicles bud off from ER which are coated with COP-2.

E. Dynamin- plays a role in clatharin dependent endocytosis by acting as a pair of molecular scissors for the newly forming vesicles which comes from the plasma membrane.