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Steps in Strand Synthesis Nick ,5' 3' exonuclease RNA primer • Primer removal uw Pol sites...
The cell must remove and replace an RNA primer with DNA, if DNA pol I was...
The cell must remove and replace an RNA primer with DNA, if DNA pol I was the only enzyme available to do this, which activities must it use? a. 3'-5' exonuclease and 5'-3' polymerase b. 5'-3' exonuclease and 5'-3' polymerase c. 5'-3' exonuclease and 3'-5' polymerase d. 3'-5' exonuclease and 3'-5' polymerase
both stranas es:COVID mation & es Question 2 DNA polymerase III (DNA pol III) is needed...
both stranas es:COVID mation & es Question 2 DNA polymerase III (DNA pol III) is needed for leading strand synthesis o lagging strand synthesis both strands D Question 3 ONE RNA primer is needed for o both strands O lagging strand synthesis leading strand synthesis Question 4 Multiple RNA primers are needed for O both strands leading strand synthesis lagging strand synthesis Question 5
Order the steps in RT-PCR: 1. Add one primer complementary to the 3' end of the...
Order the steps in RT-PCR: 1. Add one primer complementary to the 3' end of the RNA of interest. Also add the RT enzyme (reverse transcriptase), dNTPs, and a buffer with Mg2+. Incubate the reaction appropriately. 2. Degrade the RNA strand with base, leaving just the cDNA. 3. cDNA is produced, which is single-stranded and complementary to the RNA of interest. 4. Double-stranded DNA for the region of interest is produced. 5. Add two primers (one forward and one reverse)...
Why were Meselson and Stahl unable to distinguish between the dispersive and the semiconservative models following...
Why were Meselson and Stahl unable to distinguish between the dispersive and the semiconservative models following only one round of DNA replication? 2. Describe in order, the four repeating steps that repeat over and over on the discontinuous lagging strand of DNA replication and name the major proteins required to carry out each of these steps in E. coli. The first function is given . I. Function: Create RNA primer Enzyme: II. Function: Enzyme: III. Function: Enzyme: IV. Function: Enzyme:...
dentify the correct action and event for clamp loading proteins catalyzes the joining of deoxyribonucleotide 5...
dentify the correct action and event for clamp loading proteins catalyzes the joining of deoxyribonucleotide 5 -triphosphates (dNTPs) to form the growing DNA chain O an enzyme that can synthesize short fragments of RNA de novo which are complementary to the parent strand template and serve as primers for DNA replication proteins that specifically recognize and bind DNA at the junction between the primer and template proteins that bind adjacent to the clamp-loading proteins, forming a ring around the template...
help me answer these- One strand of a section of DNA Isolated from the bacterium E....
help me answer these-
One strand of a section of DNA Isolated from the bacterium E. cow reads: answer all parts 5- GTAAGCTACGGATAGG -3 A. Suppose that an mRNA is transcribed from this DNA using the complementary strand as a template meaning this is the coding strand). What will be the sequence of the mRNA in this region (make sure you label the 5 and 3' ends of the mRNA)? B. How many different peptides could potentially be made from...
1. Which of the following statements is FALSE? Helicase activity 'unwinds DNA making the double-stranded molecule...
1. Which of the following statements is FALSE? Helicase activity 'unwinds DNA making the double-stranded molecule into single strands. b. The leading strand of DNA is started by an RNA primer The lagging strand of DNA is synthesized as "Okazaki fragments", cach with its own RNA primer. DNA replication proceeds in both directions around the bacterial chromosome. DNA polymerase synthesives new DNA in one direction (3 to 5) only. 2. Which of the following would be found in eukaryotes? a....
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
both stranas es:COVID mation & es Question 2 DNA polymerase III (DNA pol III) is needed for leading strand synthesis o lagging strand synthesis both strands D Question 3 ONE RNA primer is needed for o both strands O lagging strand synthesis leading strand synthesis Question 4 Multiple RNA primers are needed for O both strands leading strand synthesis lagging strand synthesis Question 5
dentify the correct action and event for clamp loading proteins catalyzes the joining of deoxyribonucleotide 5 -triphosphates (dNTPs) to form the growing DNA chain O an enzyme that can synthesize short fragments of RNA de novo which are complementary to the parent strand template and serve as primers for DNA replication proteins that specifically recognize and bind DNA at the junction between the primer and template proteins that bind adjacent to the clamp-loading proteins, forming a ring around the template...
help me answer these-
One strand of a section of DNA Isolated from the bacterium E. cow reads: answer all parts 5- GTAAGCTACGGATAGG -3 A. Suppose that an mRNA is transcribed from this DNA using the complementary strand as a template meaning this is the coding strand). What will be the sequence of the mRNA in this region (make sure you label the 5 and 3' ends of the mRNA)? B. How many different peptides could potentially be made from...
1. Which of the following statements is FALSE? Helicase activity 'unwinds DNA making the double-stranded molecule into single strands. b. The leading strand of DNA is started by an RNA primer The lagging strand of DNA is synthesized as "Okazaki fragments", cach with its own RNA primer. DNA replication proceeds in both directions around the bacterial chromosome. DNA polymerase synthesives new DNA in one direction (3 to 5) only. 2. Which of the following would be found in eukaryotes? a....
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
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