Question

For the different regulation events listed below, indicate the enzyme or factor involved and if the result is transcriptional activation, repression, or both (either one can occur) 7. Event Enzyme / Factor Activation / Repression? Acetylation of lysine residues orn histones Methyl groups added to cytosines on DNA Proteins bind to a DNA binding domain and recruit transcriptional cofactors to the region Methyl groups added to lysine residues on histones Proteins bind to the promotor region on DNA that form a pre- initiation complex Removal of acetyl groups from lysine residues on histones Removal of methyl groups from lysine residues on histones

Answer 1

Answer:

Event	Enzyme/ Factor	Activation/ Repression
Acetylation of lysine residues on histones	Histone Acetyl Transferase (HAT)	Activation
Methyl group added to cytosines on DNA	DNA Cytosine-5- Methyltransferase	Repression
Protein binds to a DNA binding domain and recruit transcriptional cofactors to the region	Helix-turn-helix, leucine zippers, zinc fingers, helix-loop-helix, etc.	Activation
Methyl group added to lysine residues on histones	Histone Methyltransferase	Repression
Proteins bind to the promotor region on DNA that form the pre-initiation complex	RNA Pol-II, TFIIA,TFIIB,TFIIE,TFIIF,TFIIH, TBP & TAF	Activation
Removal of acetyl groups from lysine residues on histones	Histone Deacetylase	Repression
Removal of methyl group from lysine residues on histones	Histone Demethylase	Activation