Question

Based on the plot below, what is the Vmax? enzyme rate (s:1) 0 500 1000 1500 2000 Substrate concentration (M)

Answer 1

When the substrate concentration raising over the time , enzymes are performing at a high rate .When the substrate concentration reaches the saturation point , it will no longer provide the enzymatic activity. i.e The catalytic activity or enzyme conversion rate will raise at the beginning and it wil be maximum when substrate concentration reaches satuaration point. At this point the velocity at which reaction is occuring is maximum after utilization of the specific substrate concentration. Beyond that substrate concentration even if we raise the substrate concentration, it will not increase the velocity anymore. Therefore,the end velocity that they can maximum reach is known as Maximum Velocity or Vmax. .Now----

Enzyme Rate (57) 500 1000 1500 2000 substrate Concentration (MM) o. Vmax = 428

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