If given a 1.0 kbp human genome fragment that does not contain any repeated sequences. Your plan is to use it as a probe for a Southern Blot containing your friend's genomic DNA. You prepare your gel by digesting with the following restriction enzymes in the following lanes:
Lane 1: size marker
Lane 2: EcoRI
Lane 3: HindIII
Lane 4: BamHI
Your friend happens to be homozygous for the region you are probing. Does that mean you will only see one band in each lane?
Question:1 - Concerning the information above, what if you decide to conduct the same procedure on a different friend who happens to be heterozygous for the region being probed? Would you expect to see only one band per lane? Explain detailly
please answer question in Bold (1) with respect to information above.
If given a 1.0 kbp human genome fragment that does not contain any repeated sequences. Your...
You have been given a 1.0 kbp human genome fragment that does not contain any repeated sequences. Your plan is to use it as a probe for a Southern Blot containing your friend's genomic DNA. You prepare your gel by digesting with the following restriction enzymes in the following lanes: Lane 1: size marker Lane 2: EcoRI Lane 3: HindIII Lane 4: BamHI Your friend happens to be homozygous for the region you are probing. Does that mean you will...
6a) You want to use homologous recombination to generate a mouse that does not express a functional XPC gene (‘Knock-out’ or KO). To do this, you want to delete exon 10 and replace it with a gene that confers resistance to the drug Neomycin (NEO. The usual second selection with tk we discussed in class is not shown here). Using the diagram as a guide: illustrate on the diagram where you expect crossing over to occur and (below the diagram)...