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2. Gene knockout in mouse A. Outline the sequence of steps necessary to generate a gene knockout in the mouse. Be sure to include the information you need to start the procedure. B. The targeted insertion of your transgene in embryonic stem (ES) cells has a very low probability of success (roughly 1 in 10,000 cells tested). Based on what you know about molecular biology, why is the probability of success so low for this step? What feature of the procedure makes it viable despite the low probability of success at this step?

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A. Gene- kno ckoue in mouse Stebs ene atuin Electro 0910too ctam) celby. nesusant colmies (drug selechon Selecton ond ezpansion

B. Gene targeting has high chances of failure as insertion of gene at correct place is a chance event. Thus the targeted insertion is a rare event. But despite this it becomes viable step due to use of selection antibiotics.

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