Homework Answers
A. 
B. Gene targeting has high chances of failure as insertion of gene at correct place is a chance event. Thus the targeted insertion is a rare event. But despite this it becomes viable step due to use of selection antibiotics.
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2. Gene knockout in mouse A. Outline the sequence of steps necessary to generate a gene...
When attempting a targeted gene knockout using mouse embryonic stem cells, there are three possible outcomes:...
When attempting a targeted gene knockout using mouse embryonic stem cells, there are three possible outcomes: targeted knockout, ectopic insertion, and no insertion. What procedures can be used to select for cells that only have the targeted gene knockout? (9 pts) Describe why Jacob and Monod used IPTG as a synthetic inducer during their experiments investigating the genetic control of the lac operon. (6 pts) Describe the function of the CAP-cAMP system in bacteria. Why does it regulate several operons...
When attempting a targeted gene knockout using mouse embryonic stem cells, there are three possible outcomes:...
When attempting a targeted gene knockout using mouse embryonic stem cells, there are three possible outcomes: targeted knockout, ectopic insertion, and no insertion. What procedures can be used to select for cells that only have the targeted gene knockout? (9 pts) Describe why Jacob and Monod used IPTG as a synthetic inducer during their experiments investigating the genetic control of the lac operon. (6 pts) Describe the function of the CAP-cAMP system in bacteria. Why does it regulate several operons...
6a) You want to use homologous recombination to generate a mouse that does not express a functional XPC gene (‘Knock-out...
6a) You want to use homologous recombination to generate a mouse that does not express a functional XPC gene (‘Knock-out’ or KO). To do this, you want to delete exon 10 and replace it with a gene that confers resistance to the drug Neomycin (NEO. The usual second selection with tk we discussed in class is not shown here). Using the diagram as a guide: illustrate on the diagram where you expect crossing over to occur and (below the diagram)...
I really need help with these questions!! 2. Bernard Davis tested the "cross-feeding" interpretation of some...
I really need help with these questions!! 2. Bernard Davis tested the "cross-feeding" interpretation of some data that showed the phenotype of one microbe as capable of being changed by another microbe. His contribution can be summarized as: A) using one strain of microbes as food for another, and then evaluating the impact upon microbial phenotype. B) combining microbes with complementing auxotrophs in a single tube and then analyzing the cells of gene exchange. C) placing a barrier between bacterial...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...
1. According to the paper, what does lactate dehydrogenase (LDH) do and what does it allow...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
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