Homework Answers
Option 4: A proton gradient is required for ATP synthase to synthesize ATP.
Racker and Stoeckenius built an experimental artificial system consisting of membrane, a bacterial proton pump activated by light, and ATP synthase to test Peter mitchelle's chemiosmotic hypothesis of ATP synthesis, according to which proton gradient created across inner mitochondrial membrane due to pumping of protons during electron transport chain is responsible for driving ATP synthesis by the ATP synthase enzyme. Their experiments provided experimental evidence for Mitchell's hypothesis.
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Done Chapter 7 HDWK Activity Fig. 7.12 1. What is the hypothesis for Racker and Stoeckenius's...
Investigators introduce two proteins into the membrane of artificial lipid vesicles: (1) an ATP synthase isolated...
Investigators introduce two proteins into the membrane of artificial lipid vesicles: (1) an ATP synthase isolated from the mitochondria of cow heart muscle, and (2) a light-activated proton pump purified from the prokaryote Halobacterium halobium. The proteins are oriented as shown in the diagram. When ADP and P; are added to the external medium and the vesicle is exposed to light, would this system produce ATP? H LIGHT protein pump ATP synthase Choose one: O No, because no electron-transport chain...
QUESTIONS 0.1 points Save Answer What is chemiosmosis? ATP synthase rotating to add a phosphate to...
QUESTIONS 0.1 points Save Answer What is chemiosmosis? ATP synthase rotating to add a phosphate to ADP - ATP All of these contribute to chemiosmosis Proton gradient in the inner membrane space causing protons to enter into the matrix causing ATP synthase to convert ADP to ATP An electrical gradient causes protons to enter the matrix causing ATP synthase to convert ADP to ATP QUESTION 10 0.1 points Save Aswer What is substrate level phosphorylation? Using Water and CO2 to...
FUework Chapter 03. Energy. Chemical Reactions, and Cellular R i Saved Required information 1 2 The mitochondrial e...
FUework Chapter 03. Energy. Chemical Reactions, and Cellular R i Saved Required information 1 2 The mitochondrial electron transport system moves electrons from a donor molecule to a receptor molecule, which in the process drives a proton (H+) pump. In order to establish a proton gradient, the protons accumulate in Part 1 of 3 O the outer membrane the Intermembrane space 3.57 O the matrbx points O the cristae elook 3. How does the proton (H+) gradient created by the...
What process uses ATP to create glucose? O ATP synthase electron transport chain O gluconeogenesis O...
What process uses ATP to create glucose? O ATP synthase electron transport chain O gluconeogenesis O citric acid cycle Question 11 (2 points) What would expect to happen in mitochondria that have fewer cristae Increased proton motive force Decreased oxygen usage increased ATP to ADP conversion O No change in mitocondrial funciton Question 12 (2 points) What is one example of when energy molecules/electron carriers are produced during the citric acid cycle? O conversion of succinate to fumarate O addition...
What is the driving force for the generation of the ATP molecules from ADP and P:(phosphate)...
What is the driving force for the generation of the ATP molecules from ADP and P:(phosphate) molecules in presence of ATP synthase enzyme (a) the concentrations of NADH and FADH, (b) The variations of the oxidation potentials(or reduction potentials) of the Electron Transport systems (c) the concentration gradient of H' ions (d) none of these O a.(a) b.(b) O c. (c) O d. (d)
Q2. The release of ATP by the beta subunit is most directly dependent on: Select ONE...
Q2.
The release of ATP by the beta subunit is most directly
dependent on:
Select ONE option:
1. the flow of protons from the intermembrane space to the
matrix.
2. the rotation of the c-ring in the membrane.
3. the passage of electrons along the electron transport
chain.
4. a conformational change in the beta subunit.
Q3.
What defines the direction of rotation of the c-ring in the F0
complex?
(Select ALL that apply.)
1. Amino acids side chains in...
Question 1 Drag and Drop to label numbers 1-10 on the mitochondria image below. There are...
Question 1 Drag and Drop to label numbers 1-10 on the mitochondria image below. There are more than 10 labels so not all will be used. Not yet answered Points out of 10.00 Mobile Protein complex of electron P Flag question 6? carriers 8? Electron FADH FAD 1? 27 5? ADP.P 9? 10? Oxygen gas Protons pumped across membrane water Proton gradient Plastocyanin Carbon dioxide АТР PSI NADH NADPH NAD+ Protons move through ATP synthase ADP ETC D Electron gradient
Glycolysis activity 1: Metabolic pathway puzzle Try to complete the activity without using the textbook. 1....
Glycolysis activity 1: Metabolic pathway puzzle Try to complete the activity without using the textbook. 1. Slides 4 and 5 show the 10 reactions for glycolysis, but some of the molecules are missing. Draw the missing molecules in the proper place (on the handout). The missing molecules are shown on the next slide, you have to use the clues on the handout and your organic chemistry knowledge to figure out where each molecule belongs. 3. In the red dotted boxes...
Evce Monobran.UUUU 4. Phosphofructokinase is the rate-limiting enzyme that controls the rate on decreases its activity...
Evce Monobran.UUUU 4. Phosphofructokinase is the rate-limiting enzyme that controls the rate on decreases its activity upon binding ATP. ATP concentrations mus y upon binding ATP. ATP concentrations must be relatively high for this to happen. This type of control of a biochemical pathway is called There are other ways to control the rate of biochemical pathways w ntrol the rate of biochemical pathways within cells. List three other typical ways cells would control rates of biochemical pathways. of relatie...
QUESTION 1 What is the purpose of the proton motive force? A Powers the phosphorylation of...
QUESTION 1 What is the purpose of the proton motive force? A Powers the phosphorylation of ATP to ADP. B. Powers the phosphorylation of ADP to ATP. C. Powers the production of high energy electron carriers. D. Powers the ability to generate more redox reactions. QUESTION 2 What is the main mechanism of action for oxidative phosphorylation? A uses the free energy of the proton gradient generated as a result of transferring electrons from NADH or FADH2 to O2 by...
Investigators introduce two proteins into the membrane of artificial lipid vesicles: (1) an ATP synthase isolated from the mitochondria of cow heart muscle, and (2) a light-activated proton pump purified from the prokaryote Halobacterium halobium. The proteins are oriented as shown in the diagram. When ADP and P; are added to the external medium and the vesicle is exposed to light, would this system produce ATP? H LIGHT protein pump ATP synthase Choose one: O No, because no electron-transport chain...
QUESTIONS 0.1 points Save Answer What is chemiosmosis? ATP synthase rotating to add a phosphate to ADP - ATP All of these contribute to chemiosmosis Proton gradient in the inner membrane space causing protons to enter into the matrix causing ATP synthase to convert ADP to ATP An electrical gradient causes protons to enter the matrix causing ATP synthase to convert ADP to ATP QUESTION 10 0.1 points Save Aswer What is substrate level phosphorylation? Using Water and CO2 to...
FUework Chapter 03. Energy. Chemical Reactions, and Cellular R i Saved Required information 1 2 The mitochondrial electron transport system moves electrons from a donor molecule to a receptor molecule, which in the process drives a proton (H+) pump. In order to establish a proton gradient, the protons accumulate in Part 1 of 3 O the outer membrane the Intermembrane space 3.57 O the matrbx points O the cristae elook 3. How does the proton (H+) gradient created by the...
What process uses ATP to create glucose? O ATP synthase electron transport chain O gluconeogenesis O citric acid cycle Question 11 (2 points) What would expect to happen in mitochondria that have fewer cristae Increased proton motive force Decreased oxygen usage increased ATP to ADP conversion O No change in mitocondrial funciton Question 12 (2 points) What is one example of when energy molecules/electron carriers are produced during the citric acid cycle? O conversion of succinate to fumarate O addition...
What is the driving force for the generation of the ATP molecules from ADP and P:(phosphate) molecules in presence of ATP synthase enzyme (a) the concentrations of NADH and FADH, (b) The variations of the oxidation potentials(or reduction potentials) of the Electron Transport systems (c) the concentration gradient of H' ions (d) none of these O a.(a) b.(b) O c. (c) O d. (d)
Q2.
The release of ATP by the beta subunit is most directly
dependent on:
Select ONE option:
1. the flow of protons from the intermembrane space to the
matrix.
2. the rotation of the c-ring in the membrane.
3. the passage of electrons along the electron transport
chain.
4. a conformational change in the beta subunit.
Q3.
What defines the direction of rotation of the c-ring in the F0
complex?
(Select ALL that apply.)
1. Amino acids side chains in...
Question 1 Drag and Drop to label numbers 1-10 on the mitochondria image below. There are more than 10 labels so not all will be used. Not yet answered Points out of 10.00 Mobile Protein complex of electron P Flag question 6? carriers 8? Electron FADH FAD 1? 27 5? ADP.P 9? 10? Oxygen gas Protons pumped across membrane water Proton gradient Plastocyanin Carbon dioxide АТР PSI NADH NADPH NAD+ Protons move through ATP synthase ADP ETC D Electron gradient
Glycolysis activity 1: Metabolic pathway puzzle Try to complete the activity without using the textbook. 1. Slides 4 and 5 show the 10 reactions for glycolysis, but some of the molecules are missing. Draw the missing molecules in the proper place (on the handout). The missing molecules are shown on the next slide, you have to use the clues on the handout and your organic chemistry knowledge to figure out where each molecule belongs. 3. In the red dotted boxes...
Evce Monobran.UUUU 4. Phosphofructokinase is the rate-limiting enzyme that controls the rate on decreases its activity upon binding ATP. ATP concentrations mus y upon binding ATP. ATP concentrations must be relatively high for this to happen. This type of control of a biochemical pathway is called There are other ways to control the rate of biochemical pathways w ntrol the rate of biochemical pathways within cells. List three other typical ways cells would control rates of biochemical pathways. of relatie...
QUESTION 1 What is the purpose of the proton motive force? A Powers the phosphorylation of ATP to ADP. B. Powers the phosphorylation of ADP to ATP. C. Powers the production of high energy electron carriers. D. Powers the ability to generate more redox reactions. QUESTION 2 What is the main mechanism of action for oxidative phosphorylation? A uses the free energy of the proton gradient generated as a result of transferring electrons from NADH or FADH2 to O2 by...
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