Question

Observations and Results S. marcesce Nutrient Broth Nutrient Agar Slant Nutrient Agar Deep Growth (+) or (-) Orange-red pigmentation (+) or (-) Draw the distribution of growth. 1. Explain why the following steps are essential during subculturing: a. Flaming the inoculating instrument prig tg and after each inocul the neck of the tubes immedi lesor recopping

Answer 1

	nutrient broth	slant agar nutrient	deep agar nutrient
growth -/+	+ ( turbidity is observed , colonies are distributed in the broth)	+( due to agar presence , medium get solidify and colonies are observed only on the top.)	+ (due to agar presence , medium get solidify and colonies are observed only on the top)
red or orange pigment-/+	+ ( red pigment present in the pellicle ( the boundary) and at the bottom )	+ ( more red pigment observed on the top because slant increase the surface area ).	+ ( less surface area as compared to slant ,so less red pigment observed as compared to slant).
distribution	Turbidity is observed and red pigment is observed in pellicle and in the bottom	Red pigment is observed only on the slant surface due to solidification	Red pigment is observed on the ton surface

Flaming is done to sterilize the instruments use during innoculation and kill the undesired organism to prevent contamination. flaming of instruments is also done after each innoculation to prevent spreading of contamination.