Question

Part B Suppose you wanted to search for QTLs influencing fruit size in tomatoes. Describe the general structure of a QTL experiment, including the kind of tomato strains you would use, how molecular markers should be distributed in the genome, how the genetic marker alleles should differ between the two strains, and how you would use the Fi progeny in a subsequent cross to obtain information about the possible location(s) of QTLs of interest. Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Not all terms will be used. Reset Help cross-breeding homozygous One could choose two tomato strains that produce tomatoes of weights, one high, one low, and cross them to produce F1 hybrids. The parent strains should be different lower for different alleles of many DNA markers, with pure-breeding one marker located every 5 to 10 CM across the entire genome. The Fi hybrids would be at all DNA heterozygous markers. inbreeding same An F2 generation could be created by of the Fi, higher and the average tomato weight of the F2 population and of each F2 individual would be determined. A statistically heavy significant correlation between an F2 plant with a self-fertilization than average tomato weight and the tomato light genotype at a DNA marker would identify that marker as a QTL.

Answer 1

One could choose two pure-breeding tomato strains that produce tomatoes of different weights, one high, one low, and cross weignis, them to produce Fi hybrids. The parent strains should be homozygous for different alleles of many DNA markers, with one marker located every 5 to 10 cm across the entire genome. The Fi hybrids would be heterozygous at all DNA markers. An F2 generation could be created by inbreeding of the Fi, and the average tomato weight of the F2 population and of each F2 individual would be determined. A statistically significant correlation between an F2 plant with a higher than average tomato weight and the same tomato genotype at a DNA marker would identify that marker as a QTL.